Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain Rossta | Penaeus chinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus chinensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rossetta by nickel affinity chromatography | Penaeus chinensis |
Renatured (Comment) | Organism |
---|---|
reactivation of denatured enzyme AK: human cyclophilin A (CyPA) has a peptidyl-prolyl cis-trans isomerase (PPIase)-independent chaperone-like activity during enzyme AK folding. Recombinant rhCyPA can form dimers and trimers during the purification procedure, catalytically inactive recombinant cyclophilin A mutant variant Q63A has even more efficiency to suppress aggregation and improve reactivation yields of enzyme AK than the wild-type cyclophilin A, overview. Catalytically inactive cyclophilin A mutants R55A, F60A, and H126A can also act as chaperones in arginine kinase folding. The refolding is initiated by a 50fold dilution in 20 mM Tris-HCl buffer, pH 7.8, containing 10 mM 2-mercaptoethanol, in the presence or absence of 0.006 mM of the wild-type rhCyPA or its variants at 20°C | Penaeus chinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Penaeus chinensis | - |