Application | Comment | Organism |
---|---|---|
medicine | the enzyme HcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm | Haemonchus contortus |
medicine | the enzyme TcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm | Teladorsagia circumcincta |
Cloned (Comment) | Organism |
---|---|
gene HcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Haemonchus contortus |
gene TcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Teladorsagia circumcincta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
agmatine | 65% inhibition at 2 mM | Haemonchus contortus | |
agmatine | 61% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
canavanine | 81% inhibition at 2 mM | Haemonchus contortus | |
canavanine | 79% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
citrulline | 17% inhibition at 2 mM | Haemonchus contortus | |
citrulline | 21% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
Creatine | 11% inhibition at 2 mM | Haemonchus contortus | |
Creatine | 9% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
glutamate | 15% inhibition at 2 mM | Haemonchus contortus | |
glutamate | 21% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
glycine | 10% inhibition at 2 mM | Haemonchus contortus | |
glycine | 15% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
guanidine | 10% inhibition at 2 mM | Haemonchus contortus | |
guanidine | 12% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
isoleucine | 10% inhibition at 2 mM | Haemonchus contortus | |
isoleucine | 9% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
lysine | 31% inhibition at 2 mM | Haemonchus contortus | |
lysine | 3% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
nitroarginine | 30% inhibition at 2 mM | Haemonchus contortus | |
nitroarginine | 30% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
ornithine | 27% inhibition at 2 mM | Haemonchus contortus | |
ornithine | 31% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
putrescine | 15% inhibition at 2 mM | Haemonchus contortus | |
putrescine | 10% enzyme inhibition at 2 mM | Teladorsagia circumcincta | |
Urea | 10% inhibition at 2 mM | Haemonchus contortus | |
Urea | 13% enzyme inhibition at 2 mM | Teladorsagia circumcincta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-arginine | pH 7.5, 30°C, recombinant His-tagged enzyme | Teladorsagia circumcincta | |
0.35 | - |
L-arginine | pH 7.5, 30°C, recombinant His-tagged enzyme | Haemonchus contortus | |
0.8 | - |
ATP | pH 7.5, 30°C, recombinant His-tagged enzyme | Teladorsagia circumcincta | |
0.9 | - |
ATP | pH 7.5, 30°C, recombinant His-tagged enzyme | Haemonchus contortus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, results in 11% activity compared to Mg2+ | Teladorsagia circumcincta | |
Cu2+ | 2 mM, results in 3% activity compared to Mg2+ | Haemonchus contortus | |
Mg2+ | 2 mM, absolutely required, no activity without Mg2+ | Teladorsagia circumcincta | |
Mg2+ | 2 mM, absolutely required, only 2% of maximal activity without Mg2+ | Haemonchus contortus | |
Mn2+ | 2 mM, results in 39% activity compared to Mg2+ | Teladorsagia circumcincta | |
Mn2+ | 2 mM, results in 41% activity compared to Mg2+ | Haemonchus contortus | |
additional information | Zn2+ cannot substitute for Mg2+ | Teladorsagia circumcincta | |
additional information | Zn2+ cannot substitute for Mg2+ | Haemonchus contortus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
- |
Teladorsagia circumcincta |
46000 | - |
- |
Haemonchus contortus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | Teladorsagia circumcincta | - |
ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | Haemonchus contortus | - |
ADP + Nomega-phospho-L-arginine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemonchus contortus | K0DG62 | gene HcAK | - |
Teladorsagia circumcincta | K0DDQ9 | gene TcAK | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Teladorsagia circumcincta | ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | - |
Haemonchus contortus | ADP + Nomega-phospho-L-arginine | - |
r | |
GTP + L-arginine | GTP shows 11% of the activity with ATP | Haemonchus contortus | GDP + Nomega-phospho-L-arginine | - |
r | |
GTP + L-arginine | GTP shows 7% of the activity with ATP | Teladorsagia circumcincta | GDP + Nomega-phospho-L-arginine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE | Teladorsagia circumcincta |
? | x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE | Haemonchus contortus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Teladorsagia circumcincta |
30 | - |
assay at | Haemonchus contortus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Teladorsagia circumcincta |
7.5 | - |
- |
Haemonchus contortus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Teladorsagia circumcincta | |
ADP | - |
Haemonchus contortus | |
ATP | - |
Teladorsagia circumcincta | |
ATP | - |
Haemonchus contortus | |
GTP | low activity | Teladorsagia circumcincta | |
GTP | low activity | Haemonchus contortus |
General Information | Comment | Organism |
---|---|---|
evolution | phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi | Teladorsagia circumcincta |
evolution | phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi | Haemonchus contortus |