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Literature summary for 2.7.3.3 extracted from

  • Umair, S.; Knight, J.S.; Bland, R.J.; Simpson, H.V.
    Molecular and biochemical characterisation of arginine kinases in Haemonchus contortus and Teladorsagia circumcincta (2013), Exp. Parasitol., 134, 362-367.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme HcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm Haemonchus contortus
medicine the enzyme TcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm Teladorsagia circumcincta

Cloned(Commentary)

Cloned (Comment) Organism
gene HcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Haemonchus contortus
gene TcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Teladorsagia circumcincta

Inhibitors

Inhibitors Comment Organism Structure
agmatine 65% inhibition at 2 mM Haemonchus contortus
agmatine 61% enzyme inhibition at 2 mM Teladorsagia circumcincta
canavanine 81% inhibition at 2 mM Haemonchus contortus
canavanine 79% enzyme inhibition at 2 mM Teladorsagia circumcincta
citrulline 17% inhibition at 2 mM Haemonchus contortus
citrulline 21% enzyme inhibition at 2 mM Teladorsagia circumcincta
Creatine 11% inhibition at 2 mM Haemonchus contortus
Creatine 9% enzyme inhibition at 2 mM Teladorsagia circumcincta
glutamate 15% inhibition at 2 mM Haemonchus contortus
glutamate 21% enzyme inhibition at 2 mM Teladorsagia circumcincta
glycine 10% inhibition at 2 mM Haemonchus contortus
glycine 15% enzyme inhibition at 2 mM Teladorsagia circumcincta
guanidine 10% inhibition at 2 mM Haemonchus contortus
guanidine 12% enzyme inhibition at 2 mM Teladorsagia circumcincta
isoleucine 10% inhibition at 2 mM Haemonchus contortus
isoleucine 9% enzyme inhibition at 2 mM Teladorsagia circumcincta
lysine 31% inhibition at 2 mM Haemonchus contortus
lysine 3% enzyme inhibition at 2 mM Teladorsagia circumcincta
nitroarginine 30% inhibition at 2 mM Haemonchus contortus
nitroarginine 30% enzyme inhibition at 2 mM Teladorsagia circumcincta
ornithine 27% inhibition at 2 mM Haemonchus contortus
ornithine 31% enzyme inhibition at 2 mM Teladorsagia circumcincta
putrescine 15% inhibition at 2 mM Haemonchus contortus
putrescine 10% enzyme inhibition at 2 mM Teladorsagia circumcincta
Urea 10% inhibition at 2 mM Haemonchus contortus
Urea 13% enzyme inhibition at 2 mM Teladorsagia circumcincta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
L-arginine pH 7.5, 30°C, recombinant His-tagged enzyme Teladorsagia circumcincta
0.35
-
L-arginine pH 7.5, 30°C, recombinant His-tagged enzyme Haemonchus contortus
0.8
-
ATP pH 7.5, 30°C, recombinant His-tagged enzyme Teladorsagia circumcincta
0.9
-
ATP pH 7.5, 30°C, recombinant His-tagged enzyme Haemonchus contortus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 2 mM, results in 11% activity compared to Mg2+ Teladorsagia circumcincta
Cu2+ 2 mM, results in 3% activity compared to Mg2+ Haemonchus contortus
Mg2+ 2 mM, absolutely required, no activity without Mg2+ Teladorsagia circumcincta
Mg2+ 2 mM, absolutely required, only 2% of maximal activity without Mg2+ Haemonchus contortus
Mn2+ 2 mM, results in 39% activity compared to Mg2+ Teladorsagia circumcincta
Mn2+ 2 mM, results in 41% activity compared to Mg2+ Haemonchus contortus
additional information Zn2+ cannot substitute for Mg2+ Teladorsagia circumcincta
additional information Zn2+ cannot substitute for Mg2+ Haemonchus contortus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
-
Teladorsagia circumcincta
46000
-
-
Haemonchus contortus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine Teladorsagia circumcincta
-
ADP + Nomega-phospho-L-arginine
-
r
ATP + L-arginine Haemonchus contortus
-
ADP + Nomega-phospho-L-arginine
-
r

Organism

Organism UniProt Comment Textmining
Haemonchus contortus K0DG62 gene HcAK
-
Teladorsagia circumcincta K0DDQ9 gene TcAK
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine
-
Teladorsagia circumcincta ADP + Nomega-phospho-L-arginine
-
r
ATP + L-arginine
-
Haemonchus contortus ADP + Nomega-phospho-L-arginine
-
r
GTP + L-arginine GTP shows 11% of the activity with ATP Haemonchus contortus GDP + Nomega-phospho-L-arginine
-
r
GTP + L-arginine GTP shows 7% of the activity with ATP Teladorsagia circumcincta GDP + Nomega-phospho-L-arginine
-
r

Subunits

Subunits Comment Organism
? x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE Teladorsagia circumcincta
? x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE Haemonchus contortus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Teladorsagia circumcincta
30
-
assay at Haemonchus contortus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Teladorsagia circumcincta
7.5
-
-
Haemonchus contortus

Cofactor

Cofactor Comment Organism Structure
ADP
-
Teladorsagia circumcincta
ADP
-
Haemonchus contortus
ATP
-
Teladorsagia circumcincta
ATP
-
Haemonchus contortus
GTP low activity Teladorsagia circumcincta
GTP low activity Haemonchus contortus

General Information

General Information Comment Organism
evolution phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi Teladorsagia circumcincta
evolution phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi Haemonchus contortus