Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 | Myzostoma cirriferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | bisubstrate kinetics and binary dissociation constants, overview | Myzostoma cirriferum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Myzostoma cirriferum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
His-tagged recombinant enzyme, gel filtration | Myzostoma cirriferum |
37000 | - |
- |
Myzostoma cirriferum |
39600 | - |
- |
Myzostoma cirriferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | Myzostoma cirriferum | - |
ADP + Nomega-phospho-L-arginine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myzostoma cirriferum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Myzostoma cirriferum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-arginine = ADP + Nomega-phospho-L-arginine | the two-substrate AK kinetics is explained by a random-order, rapid-equilibrium, kinetic mechanism | Myzostoma cirriferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Myzostoma cirriferum | ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | the enzyme is highly specific for L-arginine, reversible transfer of phosphate | Myzostoma cirriferum | ADP + Nomega-phospho-L-arginine | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39600, about, sequence calculation, 1 * 37000, His-tagged recombinant enzyme, SDS-PAGE | Myzostoma cirriferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Myzostoma cirriferum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.9 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Myzostoma cirriferum | |
38.9 | - |
L-arginine | pH 7.5, 25°C, recombinant enzyme | Myzostoma cirriferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Myzostoma cirriferum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Myzostoma cirriferum |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of amino acid sequences of phosphagen kinases indicate that the Myzostoma AK gene lineage differs from that of the polychaete Sabellastarte spectabilis AK, which is a dimer of creatine kinase (CK) origin. It is likely that the Myzostoma AK gene lineage was lost at an early stage of annelid evolution and that Sabellastarte AK evolved secondarily from the CK gene. Analysis of evolution of phosphagen kinases of annelids with marked diversity, overview | Myzostoma cirriferum |