Cloned (Comment) | Organism |
---|---|
isoform AK2 fused to maltose-binding protein is expressed in Escherichia coli TB1 cells | Sabellastarte spectabilis |
Protein Variants | Comment | Organism |
---|---|---|
G54A | the mutant shows considerably increased catalytic efficiency for L-arginine and D-arginine compared to the wild type enzyme | Sabellastarte spectabilis |
G54I | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
G54L | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
G54S | the mutant shows increased catalytic efficiency for L-arginine and D-arginine compared to the wild type enzyme | Sabellastarte spectabilis |
G54V | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
L64A | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
L64G | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
L64I | in the mutant, the affinity for L-arginine is greatly increased (9.5fold that of the wild type), whereas its affinity for D-arginine is increased 2.9fold | Sabellastarte spectabilis |
L64V | the mutant shows a 1.7fold decrease in affinity for L-arginine, but unchanged affinity for D-arginine | Sabellastarte spectabilis |
N320A | the mutant shows considerably reduced enzymatic activity (17.1% for L-arginine and 5.19% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
N320D | the mutant shows considerably reduced enzymatic activity (32.4% for L-arginine and 48.4% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
N320E | the mutant shows considerably reduced enzymatic activity (52.3% for L-arginine and 10.1% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
N320H | the mutant shows considerably reduced enzymatic activity (29.5% for L-arginine and 10.2% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
N320K | the mutant shows considerably reduced enzymatic activity (0.612% for L-arginine and 0.489% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
N320Q | the mutant shows considerably reduced enzymatic activity (8.97% for L-arginine and 2,6% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
N320R | the mutant shows considerably reduced enzymatic activity (4.11% for L-arginine and 1.46% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
Y89Q | the mutant shows increased catalytic efficiency for L-arginine and D-arginine compared to the wild type enzyme | Sabellastarte spectabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.389 | - |
L-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
0.4 | - |
ATP | isoform AK2 mutant L64I, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
0.661 | - |
ATP | isoform AK2 mutant G54A, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
0.774 | - |
ATP | isoform AK2 mutant L64V, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
0.837 | - |
ATP | isoform AK2 mutant L64I, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
0.926 | - |
ATP | isoform AK2 mutant G54S, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
0.93 | - |
ATP | isoform AK2 mutant Y89Q, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
1.12 | - |
ATP | isoform AK2 mutant G54A, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
1.13 | - |
ATP | wild type enzyme isoform AK2, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
1.59 | - |
ATP | isoform AK2 mutant Y89Q, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.05 | - |
L-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.72 | - |
L-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.99 | - |
ATP | wild type enzyme isoform AK2, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
3.14 | - |
D-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
3.49 | - |
ATP | isoform AK2 mutant G54S, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
3.69 | - |
L-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
3.72 | - |
ATP | isoform AK2 mutant L64V, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
4.4 | - |
D-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
5.07 | - |
L-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
6.45 | - |
L-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
6.45 | - |
D-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9 | - |
D-Arg | wild type enzyme, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9.34 | - |
D-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9.55 | - |
D-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sabellastarte spectabilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Arg | isoform AK1 shows 7.6% activity with D-arginine compared to L-arginine, isoform AK2 shows 35% activity with D-arginine compared to L-arginine | Sabellastarte spectabilis | ADP + Nomega-phospho-D-Arg | - |
? | |
ATP + L-Arg | isoforms AK1 and AK2 are primarily active towards L-arginine | Sabellastarte spectabilis | ADP + Nomega-phospho-L-Arg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AK1 | isoform | Sabellastarte spectabilis |
AK2 | isoform | Sabellastarte spectabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.4 | - |
ATP | isoform AK2 mutant Y89Q, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
36.6 | - |
ATP | wild type enzyme isoform AK2, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
39.5 | - |
ATP | wild type isoform AK2, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
40.8 | - |
D-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
41 | - |
ATP | isoform AK2 mutant G54A, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
41.9 | - |
ATP | isoform AK2 mutant Y89Q, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
43.2 | - |
ATP | isoform AK2 mutant G54S, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
43.3 | - |
ATP | isoform AK2 mutant L64V, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
46 | - |
L-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
50.1 | - |
ATP | isoform AK2 mutant L64I, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
60.7 | - |
ATP | isoform AK2 mutant G54S, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
61.5 | - |
D-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
61.8 | - |
ATP | isoform AK2 mutant L64V, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
62.3 | - |
L-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
65.8 | - |
ATP | isoform AK2 mutant G54A, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
68.3 | - |
ATP | isoform AK2 mutant L64I, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
75.4 | - |
L-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
77.8 | - |
L-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
81.2 | - |
L-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
86.7 | - |
D-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
90.7 | - |
D-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
90.7 | - |
D-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
121 | - |
L-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
121 | - |
D-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.52 | - |
D-Arg | wild type enzyme, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9.29 | - |
D-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9.52 | - |
D-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9.54 | - |
D-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
9.65 | - |
L-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
12.5 | - |
L-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
15.4 | - |
L-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
27.5 | - |
D-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
29 | - |
D-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
39.5 | - |
L-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
44.7 | - |
L-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
195 | - |
L-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis |