Cloned (Comment) | Organism |
---|---|
expressed as a fusion protein with maltose-binding protein in Escherichia coli TB1 cells | Ascaris suum |
Protein Variants | Comment | Organism |
---|---|---|
A105S | the mutant shows decreased Km values and turnover number for L-Arg (about 120% affinity) and ATP as well as slightly decreased catalytic efficiency (to about 90%) compared to the wild type enzyme | Ascaris suum |
A105S/S106G | the mutant shows increased Km values and turnover number for L-Arg (about 70% affinity) and ATP as well as decreased catalytic efficiency (to about 70%) compared to the wild type enzyme | Ascaris suum |
S106G | the mutant shows increased Km values and turnover number for L-Arg (about 60% affinity) and ATP as well as decreased catalytic efficiency (to about 60%) compared to the wild type enzyme | Ascaris suum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.106 | - |
L-Arg | mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.126 | - |
L-Arg | recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.18 | - |
L-Arg | mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.211 | - |
L-Arg | mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.49 | 2 | ATP | mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.654 | - |
ATP | recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.744 | - |
ATP | mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
0.766 | - |
ATP | mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45341 | - |
x * 45341, recombinant enzyme, calculated from amino acid sequence | Ascaris suum |
85000 | - |
x * 85000, recombinant enzyme fused to maltose-binding protein, SDS-PAGE | Ascaris suum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | D5FLG2 | - |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Ascaris suum | ADP + Nomega-phospho-L-Arg | - |
r | |
additional information | no activity towards D-arginine, creatine, glycocyamine, and taurocyamine | Ascaris suum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45341, recombinant enzyme, calculated from amino acid sequence | Ascaris suum |
? | x * 85000, recombinant enzyme fused to maltose-binding protein, SDS-PAGE | Ascaris suum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34.8 | - |
L-Arg | mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
43.3 | - |
L-Arg | mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
45.1 | - |
L-Arg | mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
45.9 | - |
L-Arg | recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ascaris suum | calculated from amino acid sequence | - |
7.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
215 | - |
L-Arg | mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
241 | - |
L-Arg | mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
316 | - |
L-Arg | mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum | |
352 | - |
L-Arg | recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C | Ascaris suum |