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Literature summary for 2.7.3.3 extracted from

  • Nagataki, M.; Uda, K.; Jarilla, B.R.; Tokuhiro, S.; Wickramasinghe, S.; Suzuki, T.; Blair, D.; Agatsuma, T.
    Molecular and catalytic properties of an arginine kinase from the nematode Ascaris suum (2012), J. Helminthol., 86, 276-286.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion protein with maltose-binding protein in Escherichia coli TB1 cells Ascaris suum

Protein Variants

Protein Variants Comment Organism
A105S the mutant shows decreased Km values and turnover number for L-Arg (about 120% affinity) and ATP as well as slightly decreased catalytic efficiency (to about 90%) compared to the wild type enzyme Ascaris suum
A105S/S106G the mutant shows increased Km values and turnover number for L-Arg (about 70% affinity) and ATP as well as decreased catalytic efficiency (to about 70%) compared to the wild type enzyme Ascaris suum
S106G the mutant shows increased Km values and turnover number for L-Arg (about 60% affinity) and ATP as well as decreased catalytic efficiency (to about 60%) compared to the wild type enzyme Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.106
-
L-Arg mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.126
-
L-Arg recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.18
-
L-Arg mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.211
-
L-Arg mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.49 2 ATP mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.654
-
ATP recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.744
-
ATP mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
0.766
-
ATP mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45341
-
x * 45341, recombinant enzyme, calculated from amino acid sequence Ascaris suum
85000
-
x * 85000, recombinant enzyme fused to maltose-binding protein, SDS-PAGE Ascaris suum

Organism

Organism UniProt Comment Textmining
Ascaris suum D5FLG2
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Ascaris suum ADP + Nomega-phospho-L-Arg
-
r
additional information no activity towards D-arginine, creatine, glycocyamine, and taurocyamine Ascaris suum ?
-
?

Subunits

Subunits Comment Organism
? x * 45341, recombinant enzyme, calculated from amino acid sequence Ascaris suum
? x * 85000, recombinant enzyme fused to maltose-binding protein, SDS-PAGE Ascaris suum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34.8
-
L-Arg mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
43.3
-
L-Arg mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
45.1
-
L-Arg mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
45.9
-
L-Arg recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum

pI Value

Organism Comment pI Value Maximum pI Value
Ascaris suum calculated from amino acid sequence
-
7.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
215
-
L-Arg mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
241
-
L-Arg mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
316
-
L-Arg mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum
352
-
L-Arg recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C Ascaris suum