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Literature summary for 2.7.3.3 extracted from

  • Bragg, J.; Rajkovic, A.; Anderson, C.; Curtis, R.; Van Houten, J.; Begres, B.; Naples, C.; Snider, M.; Fraga, D.; Singer, M.
    Identification and characterization of a putative arginine kinase homolog from Myxococcus xanthus required for fruiting body formation and cell differentiation (2012), J. Bacteriol., 194, 2668-2676.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
Nomega-phospho-L-Arg at pH 8.0 and 25°C Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus Q1DA50
-
-
Myxococcus xanthus DK 1622 Q1DA50
-
-

Purification (Commentary)

Purification (Comment) Organism
MBPTrap HP column chromatography Myxococcus xanthus

Storage Stability

Storage Stability Organism
0°C, purified enzyme on ice, 24 h, 75% loss of activity Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Myxococcus xanthus ADP + Nomega-phospho-L-Arg
-
r
ATP + L-Arg
-
Myxococcus xanthus DK 1622 ADP + Nomega-phospho-L-Arg
-
r

Synonyms

Synonyms Comment Organism
ark
-
Myxococcus xanthus
MXAN2252 protein
-
Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.093
-
Nomega-phospho-L-Arg at pH 8.0 and 25°C Myxococcus xanthus

General Information

General Information Comment Organism
physiological function the putative arginine kinase from Myxococcus xanthus is required for fruiting body formation and cell differentiation Myxococcus xanthus