BRENDA - Enzyme Database
show all sequences of 2.7.3.3

Identification and characterization of a putative arginine kinase homolog from Myxococcus xanthus required for fruiting body formation and cell differentiation

Bragg, J.; Rajkovic, A.; Anderson, C.; Curtis, R.; Van Houten, J.; Begres, B.; Naples, C.; Snider, M.; Fraga, D.; Singer, M.; J. Bacteriol. 194, 2668-2676 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Myxococcus xanthus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
Organism
Organism
UniProt
Commentary
Textmining
Myxococcus xanthus
Q1DA50
-
-
Myxococcus xanthus DK 1622
Q1DA50
-
-
Purification (Commentary)
Purification (Commentary)
Organism
MBPTrap HP column chromatography
Myxococcus xanthus
Storage Stability
Storage Stability
Organism
0°C, purified enzyme on ice, 24 h, 75% loss of activity
Myxococcus xanthus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + L-Arg
-
722542
Myxococcus xanthus
ADP + Nomega-phospho-L-Arg
-
-
-
r
ATP + L-Arg
-
722542
Myxococcus xanthus DK 1622
ADP + Nomega-phospho-L-Arg
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
ark
-
Myxococcus xanthus
MXAN2252 protein
-
Myxococcus xanthus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.093
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Myxococcus xanthus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
Purification (Commentary) (protein specific)
Commentary
Organism
MBPTrap HP column chromatography
Myxococcus xanthus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, purified enzyme on ice, 24 h, 75% loss of activity
Myxococcus xanthus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + L-Arg
-
722542
Myxococcus xanthus
ADP + Nomega-phospho-L-Arg
-
-
-
r
ATP + L-Arg
-
722542
Myxococcus xanthus DK 1622
ADP + Nomega-phospho-L-Arg
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.093
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
General Information
General Information
Commentary
Organism
physiological function
the putative arginine kinase from Myxococcus xanthus is required for fruiting body formation and cell differentiation
Myxococcus xanthus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the putative arginine kinase from Myxococcus xanthus is required for fruiting body formation and cell differentiation
Myxococcus xanthus
Other publictions for EC 2.7.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)