Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus cells | Apostichopus japonicus |
Protein Variants | Comment | Organism |
---|---|---|
D57A | the mutation causes pronounced loss of activity and substrate synergism, and distinct conformational changes | Apostichopus japonicus |
D57E | most of the kinetic parameters are similar to those of wild type enzyme. A small decrease in Kd/Km and kcat/Km (L-Arg) values indicate a slight loss of substrate synergism and catalytic efficiency | Apostichopus japonicus |
Q53A | the mutation causes pronounced loss of activity and substrate synergism, and distinct conformational changes | Apostichopus japonicus |
Q53A/D57A | the changes in kinetic parameters as well as the loss of substrate synergism of the double mutant are more severe than that of Q53A and D57A single mutant enzymes. In addition, the double mutant has the lowest affinity for ATP | Apostichopus japonicus |
Q53E | most of the kinetic parameters are similar to those of wild type enzyme. A small decrease in Kd/Km and kcat/Km (L-Arg) values indicate a slight loss of substrate synergism and catalytic efficiency | Apostichopus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.413 | - |
L-Arg | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
0.421 | - |
L-Arg | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
0.578 | - |
L-Arg | mutant enzyme Q53E, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
0.744 | - |
L-Arg | mutant enzyme D57E, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
0.814 | - |
ATP | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
0.823 | - |
ATP | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
0.988 | - |
ATP | mutant enzyme Q53E, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
1.036 | - |
L-Arg | mutant enzyme Q53A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
1.132 | - |
ATP | mutant enzyme D57E, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
1.321 | - |
ATP | mutant enzyme Q53A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
1.571 | - |
L-Arg | mutant enzyme D57A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
1.631 | - |
ATP | mutant enzyme D57A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
2.726 | - |
L-Arg | mutant enzyme Q53A/D57A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
3.092 | - |
ATP | mutant enzyme Q53A/D57A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apostichopus japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Apostichopus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Apostichopus japonicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Apostichopus japonicus | ADP + Nomega-phospho-L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Apostichopus japonicus |
Synonyms | Comment | Organism |
---|---|---|
ATP: L-arginine phosphotransferase | - |
Apostichopus japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | 60 | the activity of wild type enzyme changes a little after heat treatment for 10 min at temperatures below 45°C. A steep decrease of activity is observed after 10 min between 45 and 60°C, and a complete loss of activity occurs above 65°C. The midpoint of thermal inactivation of wild type enzyme is at about 52°C | Apostichopus japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.38 | - |
L-Arg | mutant enzyme Q53A/D57A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
10.26 | - |
L-Arg | mutant enzyme D57A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
13.09 | - |
L-Arg | mutant enzyme Q53A, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
15.95 | - |
L-Arg | mutant enzyme D57E, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
23.33 | - |
L-Arg | mutant enzyme Q53E, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
25.7 | - |
L-Arg | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus | |
29.93 | - |
L-Arg | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Apostichopus japonicus |