KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
L-Arg | isoform arginine kinase 2 | Ctenocephalides felis | |
0.284 | - |
ADP | isoform arginine kinase 2 | Ctenocephalides felis | |
0.7 | - |
ADP | isoform arginine kinase 1 | Ctenocephalides felis | |
0.74 | - |
Nomega-phospho-L-Arg | isoform arginine kinase 2 | Ctenocephalides felis | |
1.27 | - |
L-Arg | isoform arginine kinase 1 | Ctenocephalides felis | |
2.31 | - |
Nomega-phospho-L-Arg | isoform arginine kinase 1 | Ctenocephalides felis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
isoform AK1, estimated from SDS-PAGE | Ctenocephalides felis |
43400 | - |
isoform AK2, estimated from SDS-PAGE | Ctenocephalides felis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ctenocephalides felis | - |
cat flea | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Ctenocephalides felis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | highest activity | Ctenocephalides felis | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-arginine-O-ethyl ester | isoform arginine kinase 2 shows about 16% activity of that obtained with L-Arg | Ctenocephalides felis | ? | - |
? | |
ATP + L-canavanine | isoform arginine kinase 1 shows 10% activity of that obtained with L-Arg, isoform arginine kinase 2 shows about 9.5% activity of that obtained with L-Arg | Ctenocephalides felis | ADP + L-phosphocanavanine | - |
? | |
ATP + L-homoarginine | isoform arginine kinase 2 shows about 37% activity of that obtained with L-Arg | Ctenocephalides felis | ADP + Nomega-phospho-L-homoarginine | - |
? | |
additional information | L-arginine-O-ethyl ester and L-homo-arginine are extremely poor substrates for isoform AK1 with only 3.5% and 2% of the L-arginine reaction rate. L-Nalpha-acetyl-arginine, agmatine, creatine, 4-guanidino butyric acid, N7-methyl-arginine and N7-nitro-arginine are no substrates for isoform AK1. Minor substrates for isoform AK2 are L-Nalpha-acetyl-arginine and 4-guanidino butyric acid with 1.5% and 1.9% of the L-arginine reaction rate, respectively. Agmatine, creatine, L-N7-methyl-arginine and L-N7-nitro-arginine are no substrates for isoform AK2 | Ctenocephalides felis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AK1 | isoform | Ctenocephalides felis |
AK2 | isoform | Ctenocephalides felis |
ATP:L-arginine N-phosphotransferase | - |
Ctenocephalides felis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ctenocephalides felis |