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Literature summary for 2.7.3.3 extracted from

  • Werr, M.; Cramer, J.; Ilg, T.
    Identification and characterization of two arginine kinases from the parasitic insect Ctenocephalides felis (2009), Insect Biochem. Mol. Biol., 39, 634-645.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
L-Arg isoform arginine kinase 2 Ctenocephalides felis
0.284
-
ADP isoform arginine kinase 2 Ctenocephalides felis
0.7
-
ADP isoform arginine kinase 1 Ctenocephalides felis
0.74
-
Nomega-phospho-L-Arg isoform arginine kinase 2 Ctenocephalides felis
1.27
-
L-Arg isoform arginine kinase 1 Ctenocephalides felis
2.31
-
Nomega-phospho-L-Arg isoform arginine kinase 1 Ctenocephalides felis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
isoform AK1, estimated from SDS-PAGE Ctenocephalides felis
43400
-
isoform AK2, estimated from SDS-PAGE Ctenocephalides felis

Organism

Organism UniProt Comment Textmining
Ctenocephalides felis
-
cat flea
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Ctenocephalides felis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg highest activity Ctenocephalides felis ADP + Nomega-phospho-L-Arg
-
?
ATP + L-arginine-O-ethyl ester isoform arginine kinase 2 shows about 16% activity of that obtained with L-Arg Ctenocephalides felis ?
-
?
ATP + L-canavanine isoform arginine kinase 1 shows 10% activity of that obtained with L-Arg, isoform arginine kinase 2 shows about 9.5% activity of that obtained with L-Arg Ctenocephalides felis ADP + L-phosphocanavanine
-
?
ATP + L-homoarginine isoform arginine kinase 2 shows about 37% activity of that obtained with L-Arg Ctenocephalides felis ADP + Nomega-phospho-L-homoarginine
-
?
additional information L-arginine-O-ethyl ester and L-homo-arginine are extremely poor substrates for isoform AK1 with only 3.5% and 2% of the L-arginine reaction rate. L-Nalpha-acetyl-arginine, agmatine, creatine, 4-guanidino butyric acid, N7-methyl-arginine and N7-nitro-arginine are no substrates for isoform AK1. Minor substrates for isoform AK2 are L-Nalpha-acetyl-arginine and 4-guanidino butyric acid with 1.5% and 1.9% of the L-arginine reaction rate, respectively. Agmatine, creatine, L-N7-methyl-arginine and L-N7-nitro-arginine are no substrates for isoform AK2 Ctenocephalides felis ?
-
?

Synonyms

Synonyms Comment Organism
AK1 isoform Ctenocephalides felis
AK2 isoform Ctenocephalides felis
ATP:L-arginine N-phosphotransferase
-
Ctenocephalides felis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Ctenocephalides felis