Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes (Y75F, Y75D, P272G, P272R, Y75F/P272G and Y75D/P272R) in Escherichia coli BL21 (DE3) | Locusta migratoria manilensis |
Protein Variants | Comment | Organism |
---|---|---|
P272G | the synergism is almost completely lost. Fluorescence spectrum shows a red shift | Locusta migratoria manilensis |
P272R | the synergism is almost completely lost. Fluorescence spectrum shows a red shift | Locusta migratoria manilensis |
Y75D | mutant shows strong synergism. Fluorescence spectrum shows a red shift | Locusta migratoria manilensis |
Y75D/P272R | characteristics similar to those of the wild-type enzyme | Locusta migratoria manilensis |
Y75F | mutant shows strong synergism. Fluorescence spectrum shows a red shift | Locusta migratoria manilensis |
Y75F/P272G | the synergism is almost completely lost. Fluorescence spectrum shows a red shift | Locusta migratoria manilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
L-Arg | pH 8.6, 30°C, mutant enzyme Y75D | Locusta migratoria manilensis | |
0.94 | - |
L-Arg | pH 8.6, 30°C, mutant enzyme Y75F | Locusta migratoria manilensis | |
0.94 | - |
L-Arg | pH 8.6, 30°C, native arginine kinase | Locusta migratoria manilensis | |
0.95 | - |
L-Arg | pH 8.6, 30°C, recombinant arginine kinase | Locusta migratoria manilensis | |
1.099 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75F | Locusta migratoria manilensis | |
1.27 | - |
ATP | pH 8.6, 30°C, recombinant arginine kinase | Locusta migratoria manilensis | |
1.29 | - |
ATP | pH 8.6, 30°C, native arginine kinase | Locusta migratoria manilensis | |
1.36 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75D | Locusta migratoria manilensis | |
1.53 | - |
L-Arg | pH 8.6, 30°C, mutant enzyme Y75D/P272R | Locusta migratoria manilensis | |
1.65 | - |
ATP | pH 8.6, 30°C, mutant enzyme P272G | Locusta migratoria manilensis | |
1.93 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75D/P272R | Locusta migratoria manilensis | |
2.84 | - |
ATP | pH 8.6, 30°C, mutant enzyme P272R | Locusta migratoria manilensis | |
2.85 | - |
L-Arg | pH 8.6, 30°C, mutant enzyme P272R | Locusta migratoria manilensis | |
3.78 | - |
L-Arg | pH 8.6, 30°C, mutant enzyme P272G | Locusta migratoria manilensis | |
3.98 | - |
L-Arg | pH 8.6, 30°C, mutant enzyme Y75F/P272G | Locusta migratoria manilensis | |
4.2 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75F/P272G | Locusta migratoria manilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria manilensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | synergism in substrate binding | Locusta migratoria manilensis | ADP + omega-N-phospho-L-Arg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.3 | - |
ATP | pH 8.6, 30°C, mutant enzyme P272R | Locusta migratoria manilensis | |
74.6 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75F/P272G | Locusta migratoria manilensis | |
79.2 | - |
ATP | pH 8.6, 30°C, mutant enzyme P272G | Locusta migratoria manilensis | |
126.2 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75F | Locusta migratoria manilensis | |
140.7 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75D | Locusta migratoria manilensis | |
141.3 | - |
ATP | pH 8.6, 30°C, mutant enzyme Y75D/P272R | Locusta migratoria manilensis | |
159.4 | - |
ATP | pH 8.6, 30°C, recombinant arginine kinase | Locusta migratoria manilensis | |
163 | - |
ATP | pH 8.6, 30°C, native arginine kinase | Locusta migratoria manilensis |