Cloned (Comment) | Organism |
---|---|
expressed as fusion proteins with the maltose-binding protein in Escherichia coli. When expressed alone, domain 1 displays minimal activity. When expressed alone, domain 2 has significantly higher activity and catalytic efficiency that the two-domain wild-type AK, when domain 1 is inactivated using the Y68A mutation, activity is about 50% of the wild-type enzyme and when domain 2 is inactivated using the Y68A mutation, activity is retained at about 12% of the wild-type level | Abyssogena kaikoi |
Protein Variants | Comment | Organism |
---|---|---|
Y68A | introduction of a Y68A mutation in both domains virtually abolishes catalytic activity. Significant residual activity is observed, when the Y68A mutation is introduced only into domain 2 of the two-domain enzyme. A similar mutation in domain 1 of the two domain enzyme reduces activity to a much lower extent | Abyssogena kaikoi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.912 | - |
L-arginine | wild-type two-domain enzyme | Abyssogena kaikoi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
calculated from sequence | Abyssogena kaikoi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abyssogena kaikoi | Q4AEC4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | both domains of Calyptogena arginine kinase are catalytically competent, although domain 2 strongly influences catalysis in domain 1 | Abyssogena kaikoi | ADP + omega-N-phospho-L-arginine | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.9 | - |
ATP | wild-type two-domain enzyme | Abyssogena kaikoi | |
212.9 | - |
L-arginine | wild-type two-domain enzyme | Abyssogena kaikoi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Abyssogena kaikoi | calculated from sequence | - |
6.7 |