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Literature summary for 2.7.3.3 extracted from

  • Uda, K.; Yamamoto, K.; Iwasaki, N.; Iwai, M.; Fujikura, K.; Ellington, W.R.; Suzuki, T.
    Two-domain arginine kinase from the deep-sea clam Calyptogena kaikoi - evidence of two active domains (2008), Comp. Biochem. Physiol. B, 151, 176-182.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as fusion proteins with the maltose-binding protein in Escherichia coli. When expressed alone, domain 1 displays minimal activity. When expressed alone, domain 2 has significantly higher activity and catalytic efficiency that the two-domain wild-type AK, when domain 1 is inactivated using the Y68A mutation, activity is about 50% of the wild-type enzyme and when domain 2 is inactivated using the Y68A mutation, activity is retained at about 12% of the wild-type level Abyssogena kaikoi

Protein Variants

Protein Variants Comment Organism
Y68A introduction of a Y68A mutation in both domains virtually abolishes catalytic activity. Significant residual activity is observed, when the Y68A mutation is introduced only into domain 2 of the two-domain enzyme. A similar mutation in domain 1 of the two domain enzyme reduces activity to a much lower extent Abyssogena kaikoi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.912
-
L-arginine wild-type two-domain enzyme Abyssogena kaikoi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
calculated from sequence Abyssogena kaikoi

Organism

Organism UniProt Comment Textmining
Abyssogena kaikoi Q4AEC4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine both domains of Calyptogena arginine kinase are catalytically competent, although domain 2 strongly influences catalysis in domain 1 Abyssogena kaikoi ADP + omega-N-phospho-L-arginine
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.9
-
ATP wild-type two-domain enzyme Abyssogena kaikoi
212.9
-
L-arginine wild-type two-domain enzyme Abyssogena kaikoi

pI Value

Organism Comment pI Value Maximum pI Value
Abyssogena kaikoi calculated from sequence
-
6.7