Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, structure of ligand-free TcAK is determined by molecular replacement methods and refined at 1.9 A resolution | Trypanosoma cruzi |
hanging drop vapour diffusion method using 2.5 M ammonium sulfate, and 0.1 M Tris-HCl, pH 7.5 | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | O96507 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-column chromatography and Superdex-75 gel filtration | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
TcAK | - |
Trypanosoma cruzi |