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Literature summary for 2.7.3.3 extracted from

  • Uda, K.; Suzuki, T.
    A novel arginine kinase with substrate specificity towards D-arginine (2007), Protein J., 26, 281-291.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of cDNAs into pMAL plasmid and expression in Escherichia coli as a fusion protein with MBP tag or hexameric His tag Sabellastarte spectabilis
expressed in Escherichia coli Sabellastarte spectabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
L-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis
13.9
-
D-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis
34.3
-
L-Arg 25°C Sabellastarte spectabilis
35.4
-
L-Arg 25°C Sabellastarte spectabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, Superdex 75 gel filtration Sabellastarte spectabilis
42273
-
2 * 42273, calculated from sequence Sabellastarte spectabilis
42887
-
2 * 42887, calculated from sequence Sabellastarte spectabilis
42890
-
calculated from sequence of cDNA Sabellastarte spectabilis
80000
-
fusion proteins with hexameric His tag, gel filtration Sabellastarte spectabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-Arg Sabellastarte spectabilis
-
ADP + omega-N-phospho-D-Arg
-
?

Organism

Organism UniProt Comment Textmining
Sabellastarte spectabilis
-
-
-
Sabellastarte spectabilis Q4AED1
-
-
Sabellastarte spectabilis Q4AED2
-
-

Purification (Commentary)

Purification (Comment) Organism
Amberlite IR120BNA column chromatography and reverse-phase high performance liquid chromatography Sabellastarte spectabilis

Source Tissue

Source Tissue Comment Organism Textmining
body wall muscle
-
Sabellastarte spectabilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-Arg 35.2% relative activity compared to L-Arg Sabellastarte spectabilis ADP + Nomega-phospho-D-Arg
-
?
ATP + D-Arg 35.2% of the activity with L-Arg Sabellastarte spectabilis ADP + Nomega-phospho-D-Arg
-
?
ATP + D-Arg
-
Sabellastarte spectabilis ADP + omega-N-phospho-D-Arg
-
?
ATP + D-Arg 7.6% of the activity with L-Arg Sabellastarte spectabilis ADP + omega-N-phospho-D-Arg
-
?
ATP + L-Arg
-
Sabellastarte spectabilis ADP + Nomega-phospho-L-Arg
-
?
ATP + L-Arg 100% activity Sabellastarte spectabilis ADP + Nomega-phospho-L-Arg
-
?
ATP + L-Arg
-
Sabellastarte spectabilis ADP + omega-N-phospho-L-Arg
-
?
ATP + lombricine 0.17% of the activity with L-Arg Sabellastarte spectabilis ADP + omega-N-phospholombricine
-
?
ATP + lombricine 3.0% of the activity with L-Arg Sabellastarte spectabilis ADP + omega-N-phospholombricine
-
?
ATP + taurocyamine 0.11% of the activity with L-Arg Sabellastarte spectabilis ADP + N-phosphotaurocyamine
-
?
ATP + taurocyamine 2.7% of the activity with L-Arg Sabellastarte spectabilis ADP + N-phosphotaurocyamine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, Superdex 75 gel filtration Sabellastarte spectabilis
dimer 2 * 42273, calculated from sequence Sabellastarte spectabilis
dimer 2 * 42887, calculated from sequence Sabellastarte spectabilis

Synonyms

Synonyms Comment Organism
AK1
-
Sabellastarte spectabilis
AK2
-
Sabellastarte spectabilis
arginine kinase 1
-
Sabellastarte spectabilis
arginine kinase 2
-
Sabellastarte spectabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 D-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis
5.8
-
L-Arg 25°C Sabellastarte spectabilis
35.4
-
L-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Sabellastarte spectabilis

pI Value

Organism Comment pI Value Maximum pI Value
Sabellastarte spectabilis calculated from sequence
-
4.2
Sabellastarte spectabilis calculated from sequence of cDNA
-
6.21
Sabellastarte spectabilis calculated from sequence
-
7.2