Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | protects arginine kinase from inactivation losing its native tertiary conformation and aggregation in the presence of guanidine hydrochloride | Locusta migratoria manilensis | |
glycerol | protects arginine kinase from inactivation in both low and high concentrations of guanidine hydrochloride | Locusta migratoria manilensis | |
proline | with proline the residual activity of AK after incubation in guanidine hydrochloride is higher than without proline | Locusta migratoria manilensis | |
sucrose | maintains the activity of the enzyme in the presence of guanidine hydrochloride | Locusta migratoria manilensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | 1 mM | Locusta migratoria manilensis |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | prevents arginine kinase unfolding and inactivation that is due to a guanidine hydrochloride denaturant by reducing the inactivation rate constants, increasing the transition free energy changes and increasing the value for the midpoint of denaturation | Locusta migratoria manilensis |
Glycerol | prevents arginine kinase unfolding and inactivation that is due to a guanidine hydrochloride denaturant by reducing the inactivation rate constants, increasing the transition free energy changes and increasing the value for the midpoint of denaturation | Locusta migratoria manilensis |
guanidine-HCl | 2.0 M guanidine hydrochloride results in almost complete unfolding of arginine kinase. When adding the osmolytes (Pro, sucrose, DMSO, glycerol) to the unfolding system, 2.0 M guanidine hydrochloride only brings about a partial red shift compared to the samples without osmolyte. Osmolytes stabilize the arginine kinase conformation against guanidine hydrochloride in a concentration-dependent manner | Locusta migratoria manilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria manilensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Locusta migratoria manilensis |
Sephacryl S-200HR gel filtration and DEAE Sepharose CL-6B fast flow chromatography | Locusta migratoria manilensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Locusta migratoria manilensis | - |
muscle | leg muscle | Locusta migratoria manilensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-Arg | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ATP:L-arginine phosphotransferase | - |
Locusta migratoria manilensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Locusta migratoria manilensis |