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Literature summary for 2.7.3.3 extracted from

  • Xia, Y.; Park, Y.D.; Mu, H.; Zhou, H.M.; Wang, X.Y.; Meng, F.G.
    The protective effects of osmolytes on arginine kinase unfolding and aggregation (2007), Int. J. Biol. Macromol., 40, 437-443.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DMSO protects arginine kinase from inactivation losing its native tertiary conformation and aggregation in the presence of guanidine hydrochloride Locusta migratoria manilensis
glycerol protects arginine kinase from inactivation in both low and high concentrations of guanidine hydrochloride Locusta migratoria manilensis
proline with proline the residual activity of AK after incubation in guanidine hydrochloride is higher than without proline Locusta migratoria manilensis
sucrose maintains the activity of the enzyme in the presence of guanidine hydrochloride Locusta migratoria manilensis

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride 1 mM Locusta migratoria manilensis

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO prevents arginine kinase unfolding and inactivation that is due to a guanidine hydrochloride denaturant by reducing the inactivation rate constants, increasing the transition free energy changes and increasing the value for the midpoint of denaturation Locusta migratoria manilensis
Glycerol prevents arginine kinase unfolding and inactivation that is due to a guanidine hydrochloride denaturant by reducing the inactivation rate constants, increasing the transition free energy changes and increasing the value for the midpoint of denaturation Locusta migratoria manilensis
guanidine-HCl 2.0 M guanidine hydrochloride results in almost complete unfolding of arginine kinase. When adding the osmolytes (Pro, sucrose, DMSO, glycerol) to the unfolding system, 2.0 M guanidine hydrochloride only brings about a partial red shift compared to the samples without osmolyte. Osmolytes stabilize the arginine kinase conformation against guanidine hydrochloride in a concentration-dependent manner Locusta migratoria manilensis

Organism

Organism UniProt Comment Textmining
Locusta migratoria manilensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Locusta migratoria manilensis
Sephacryl S-200HR gel filtration and DEAE Sepharose CL-6B fast flow chromatography Locusta migratoria manilensis

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Locusta migratoria manilensis
-
muscle leg muscle Locusta migratoria manilensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Locusta migratoria manilensis ADP + Nomega-phospho-L-Arg
-
r

Synonyms

Synonyms Comment Organism
ATP:L-arginine phosphotransferase
-
Locusta migratoria manilensis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Locusta migratoria manilensis