Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Apostichopus japonicus |
Protein Variants | Comment | Organism |
---|---|---|
E314D | significantly decreased activity | Apostichopus japonicus |
E314Q | significantly decreased activity | Apostichopus japonicus |
E314V | significantly decreased activity | Apostichopus japonicus |
F315A | modest AK activity | Apostichopus japonicus |
F315H | modest AK activity | Apostichopus japonicus |
F315Y | modest AK activity | Apostichopus japonicus |
S312G/E314V/F315D/E317A/S318A/G321S | slight arginine kinase activity | Apostichopus japonicus |
S312R/F315H/V319E | modest AK activity | Apostichopus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
L-Arg | wild type enzyme | Apostichopus japonicus | |
0.84 | - |
L-Arg | mutant enzyme F315Y | Apostichopus japonicus | |
0.99 | - |
L-Arg | mutant enzyme S312R/F315H/V319E | Apostichopus japonicus | |
1.04 | - |
ATP | wild type enzyme | Apostichopus japonicus | |
1.13 | - |
L-Arg | mutant enzyme F315H | Apostichopus japonicus | |
1.49 | - |
ATP | mutant enzyme F315Y | Apostichopus japonicus | |
1.49 | - |
ATP | mutant enzyme S312R/F315H/V319E | Apostichopus japonicus | |
2.08 | - |
ATP | mutant enzyme F315H | Apostichopus japonicus | |
2.67 | - |
L-Arg | mutant enzyme F315A | Apostichopus japonicus | |
2.76 | - |
ATP | mutant enzyme F315A | Apostichopus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apostichopus japonicus | - |
sea cucumber | - |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid column chromatography and Superdex 200HR 10/30 gel filtration | Apostichopus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Apostichopus japonicus | ADP + Nomega-phospho-L-Arg | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Apostichopus japonicus |
Synonyms | Comment | Organism |
---|---|---|
ATP: arginine N-phosphotransferase | - |
Apostichopus japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-Arg | mutant enzyme S312G/E314V/F315D/E317A/S318A/G321S | Apostichopus japonicus | |
0.833 | - |
L-Arg | mutant enzyme F315Y | Apostichopus japonicus | |
1.02 | - |
L-Arg | mutant enzyme F315A | Apostichopus japonicus | |
1.1 | - |
L-Arg | wild type enzyme | Apostichopus japonicus | |
3.97 | - |
L-Arg | mutant enzyme F315H | Apostichopus japonicus | |
5.54 | - |
L-Arg | mutant enzyme S312R/F315H/V319E | Apostichopus japonicus | |
15.67 | - |
L-Arg | mutant enzyme F315Y | Apostichopus japonicus | |
18.66 | - |
L-Arg | wild type enzyme | Apostichopus japonicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Apostichopus japonicus |