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Literature summary for 2.7.3.3 extracted from

  • Zhang, J.W.; Zhao, T.J.; Wang, S.L.; Guo, Q.; Liu, T.T.; Zhao, F.; Wang, X.C.
    The roles of C-terminal loop residues of dimeric arginine kinase from sea cucumber Stichopus japonicus in catalysis, specificity and structure (2006), Int. J. Biol. Macromol., 38, 203-210.
    View publication on PubMed
Search for more literature for 2.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Apostichopus japonicus

Protein Variants

Protein Variants Comment Organism
E314D significantly decreased activity Apostichopus japonicus
E314Q significantly decreased activity Apostichopus japonicus
E314V significantly decreased activity Apostichopus japonicus
F315A modest AK activity Apostichopus japonicus
F315H modest AK activity Apostichopus japonicus
F315Y modest AK activity Apostichopus japonicus
S312G/E314V/F315D/E317A/S318A/G321S slight arginine kinase activity Apostichopus japonicus
S312R/F315H/V319E modest AK activity Apostichopus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 L-Arg wild type enzyme Apostichopus japonicus
0.84
-
 L-Arg mutant enzyme F315Y Apostichopus japonicus
0.99
-
 L-Arg mutant enzyme S312R/F315H/V319E Apostichopus japonicus
1.04
-
 ATP wild type enzyme Apostichopus japonicus
1.13
-
 L-Arg mutant enzyme F315H Apostichopus japonicus
1.49
-
 ATP mutant enzyme F315Y Apostichopus japonicus
1.49
-
 ATP mutant enzyme S312R/F315H/V319E Apostichopus japonicus
2.08
-
 ATP mutant enzyme F315H Apostichopus japonicus
2.67
-
 L-Arg mutant enzyme F315A Apostichopus japonicus
2.76
-
 ATP mutant enzyme F315A Apostichopus japonicus

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
 sea cucumber
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid column chromatography and Superdex 200HR 10/30 gel filtration Apostichopus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
 Apostichopus japonicus ADP + Nomega-phospho-L-Arg
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Apostichopus japonicus

Synonyms

Synonyms Comment Organism
ATP: arginine N-phosphotransferase
-
 Apostichopus japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 L-Arg mutant enzyme S312G/E314V/F315D/E317A/S318A/G321S Apostichopus japonicus
0.833
-
 L-Arg mutant enzyme F315Y Apostichopus japonicus
1.02
-
 L-Arg mutant enzyme F315A Apostichopus japonicus
1.1
-
 L-Arg wild type enzyme Apostichopus japonicus
3.97
-
 L-Arg mutant enzyme F315H Apostichopus japonicus
5.54
-
 L-Arg mutant enzyme S312R/F315H/V319E Apostichopus japonicus
15.67
-
 L-Arg mutant enzyme F315Y Apostichopus japonicus
18.66
-
 L-Arg wild type enzyme Apostichopus japonicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Apostichopus japonicus