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Literature summary for 2.7.3.3 extracted from

  • Wickramasinghe, S.; Uda, K.; Nagataki, M.; Yatawara, L.; Rajapakse, R.P.; Watanabe, Y.; Suzuki, T.; Agatsuma, T.
    Toxocara canis: molecular cloning, characterization, expression and comparison of the kinetics of cDNA-derived arginine kinase (2007), Exp. Parasitol., 117, 124-132.
    View publication on PubMed

Application

Application Comment Organism
medicine this enzyme as a possible novel chemotherapy target for VLM syndrome in humans Toxocara canis

Cloned(Commentary)

Cloned (Comment) Organism
cloned it in pMAL plasmid and expressed it in Escherichia coli as a fusion protein with maltose-binding protein Toxocara canis
expressed in Escherichia coli as a fusion protein with maltose-binding protein Toxocara canis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-Arg
-
Toxocara canis
0.12
-
L-Arg in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C Toxocara canis
0.3
-
ATP
-
Toxocara canis
0.3
-
ATP in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C Toxocara canis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol presence of a signal targeting peptide presumably targeting this protein to cytosol or endoplasmic reticulum Toxocara canis 5829
-
endoplasmic reticulum presence of a signal targeting peptide presumably targeting this protein to cytosol or endoplasmic reticulum Toxocara canis 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45376
-
x * 45376, calculated from sequence Toxocara canis
45380
-
calculated from sequence of cDNA Toxocara canis

Organism

Organism UniProt Comment Textmining
Toxocara canis A7YVI5
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Toxocara canis

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
Toxocara canis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.3
-
-
Toxocara canis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Toxocara canis ADP + Nomega-phospho-L-Arg
-
?
ATP + L-Arg synergism for substrate binding Toxocara canis ADP + omega-N-phospho-L-Arg
-
r
additional information very little activity for 9.5 mM D-arginine and no activity for creatine, glycocyamine, and taurocyamine substrates examined at the final substrate concentration of 2.38 mM Toxocara canis ?
-
?

Subunits

Subunits Comment Organism
? x * 45376, calculated from sequence Toxocara canis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.18
-
ATP
-
Toxocara canis
29.18
-
L-Arg
-
Toxocara canis
81
-
L-Arg in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C Toxocara canis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Toxocara canis

pI Value

Organism Comment pI Value Maximum pI Value
Toxocara canis calculated from sequence
-
8.28
Toxocara canis calculated from sequence of cDNA
-
8.38