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Literature summary for 2.7.3.3 extracted from

  • Uda, K.; Suzuki, T.
    Role of amino acid residues on the GS region of Stichopus arginine kinase and Danio creatine kinase (2004), Protein J., 23, 53-64.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D62G 2.3fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 3.5% of the wild-type value Apostichopus japonicus
D62_F63delinsDGF 2.9fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 0.01% of the wild-type value Apostichopus japonicus
delN56_V58 inactive mutant enzyme Apostichopus japonicus
E314V turnover number is 0.000019% of the wild-type value Apostichopus japonicus
F63G 20.48fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 12.9% of the wild-type value Apostichopus japonicus
H64G 1.53fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 70.4% of the wild-type value Apostichopus japonicus
L65G turnover number is 0.0011% of the wild-type value Apostichopus japonicus
R193G 3.5fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 1.7% of the wild-type value Apostichopus japonicus
S282G 2.81fold increase in KM-value for L-arginine compared to wild-type value, 16.34fold increase in KM-value for ATP compared to wild-type value, turnover number is 88.7% of the wild-type value Apostichopus japonicus
Y89R 1.35fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 35.9% of the wild-type value Apostichopus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.307
-
L-arginine mutant enzyme Y89R Apostichopus japonicus
0.413
-
L-arginine wild-type enzyme Apostichopus japonicus
0.63
-
L-arginine mutant enzyme H64G Apostichopus japonicus
0.814
-
ATP wild-type enzyme Apostichopus japonicus
0.965
-
L-arginine mutant enzyme D62G Apostichopus japonicus
1.16
-
L-arginine mutant enzyme S282G Apostichopus japonicus
1.196
-
L-arginine mutant enzyme D62_F63delinsDGF Apostichopus japonicus
1.46
-
L-arginine mutant enzyme R193G Apostichopus japonicus
8.458
-
L-arginine mutant enzyme F63G Apostichopus japonicus
13.3
-
ATP mutant enzyme S282G Apostichopus japonicus

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine
-
Apostichopus japonicus ADP + Nomega-phosphono-L-arginine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00229
-
ATP mutant enzyme L65G Apostichopus japonicus
0.00229
-
L-arginine mutant enzyme L65G Apostichopus japonicus
0.005
-
ATP mutant enzyme E314V Apostichopus japonicus
0.005
-
L-arginine mutant enzyme E314V Apostichopus japonicus
0.0329
-
ATP mutant enzyme D62_F63delinsDGF Apostichopus japonicus
0.0329
-
L-arginine mutant enzyme D62_F63delinsDGF Apostichopus japonicus
0.443
-
ATP mutant enzyme R193G Apostichopus japonicus
0.443
-
L-arginine mutant enzyme R193G Apostichopus japonicus
0.913
-
ATP mutant enzyme D62G Apostichopus japonicus
0.913
-
L-arginine mutant enzyme D62G Apostichopus japonicus
3.31
-
ATP mutant enzyme F63G Apostichopus japonicus
3.31
-
L-arginine mutant enzyme F63G Apostichopus japonicus
9.22
-
ATP mutant enzyme Y89R Apostichopus japonicus
9.22
-
L-arginine mutant enzyme Y89R Apostichopus japonicus
18.09
-
ATP mutant enzyme H64G Apostichopus japonicus
18.09
-
L-arginine mutant enzyme H64G Apostichopus japonicus
22.8
-
ATP mutant enzyme S282G Apostichopus japonicus
22.8
-
L-arginine mutant enzyme S282G Apostichopus japonicus
25.7
-
ATP wild-type enzyme Apostichopus japonicus
25.7
-
L-arginine wild-type enzyme Apostichopus japonicus