Protein Variants | Comment | Organism |
---|---|---|
D62G | 2.3fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 3.5% of the wild-type value | Apostichopus japonicus |
D62_F63delinsDGF | 2.9fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 0.01% of the wild-type value | Apostichopus japonicus |
delN56_V58 | inactive mutant enzyme | Apostichopus japonicus |
E314V | turnover number is 0.000019% of the wild-type value | Apostichopus japonicus |
F63G | 20.48fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 12.9% of the wild-type value | Apostichopus japonicus |
H64G | 1.53fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 70.4% of the wild-type value | Apostichopus japonicus |
L65G | turnover number is 0.0011% of the wild-type value | Apostichopus japonicus |
R193G | 3.5fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 1.7% of the wild-type value | Apostichopus japonicus |
S282G | 2.81fold increase in KM-value for L-arginine compared to wild-type value, 16.34fold increase in KM-value for ATP compared to wild-type value, turnover number is 88.7% of the wild-type value | Apostichopus japonicus |
Y89R | 1.35fold increase in KM-value for L-arginine compared to wild-type value, turnover number is 35.9% of the wild-type value | Apostichopus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.307 | - |
L-arginine | mutant enzyme Y89R | Apostichopus japonicus | |
0.413 | - |
L-arginine | wild-type enzyme | Apostichopus japonicus | |
0.63 | - |
L-arginine | mutant enzyme H64G | Apostichopus japonicus | |
0.814 | - |
ATP | wild-type enzyme | Apostichopus japonicus | |
0.965 | - |
L-arginine | mutant enzyme D62G | Apostichopus japonicus | |
1.16 | - |
L-arginine | mutant enzyme S282G | Apostichopus japonicus | |
1.196 | - |
L-arginine | mutant enzyme D62_F63delinsDGF | Apostichopus japonicus | |
1.46 | - |
L-arginine | mutant enzyme R193G | Apostichopus japonicus | |
8.458 | - |
L-arginine | mutant enzyme F63G | Apostichopus japonicus | |
13.3 | - |
ATP | mutant enzyme S282G | Apostichopus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apostichopus japonicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Apostichopus japonicus | ADP + Nomega-phosphono-L-arginine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00229 | - |
ATP | mutant enzyme L65G | Apostichopus japonicus | |
0.00229 | - |
L-arginine | mutant enzyme L65G | Apostichopus japonicus | |
0.005 | - |
ATP | mutant enzyme E314V | Apostichopus japonicus | |
0.005 | - |
L-arginine | mutant enzyme E314V | Apostichopus japonicus | |
0.0329 | - |
ATP | mutant enzyme D62_F63delinsDGF | Apostichopus japonicus | |
0.0329 | - |
L-arginine | mutant enzyme D62_F63delinsDGF | Apostichopus japonicus | |
0.443 | - |
ATP | mutant enzyme R193G | Apostichopus japonicus | |
0.443 | - |
L-arginine | mutant enzyme R193G | Apostichopus japonicus | |
0.913 | - |
ATP | mutant enzyme D62G | Apostichopus japonicus | |
0.913 | - |
L-arginine | mutant enzyme D62G | Apostichopus japonicus | |
3.31 | - |
ATP | mutant enzyme F63G | Apostichopus japonicus | |
3.31 | - |
L-arginine | mutant enzyme F63G | Apostichopus japonicus | |
9.22 | - |
ATP | mutant enzyme Y89R | Apostichopus japonicus | |
9.22 | - |
L-arginine | mutant enzyme Y89R | Apostichopus japonicus | |
18.09 | - |
ATP | mutant enzyme H64G | Apostichopus japonicus | |
18.09 | - |
L-arginine | mutant enzyme H64G | Apostichopus japonicus | |
22.8 | - |
ATP | mutant enzyme S282G | Apostichopus japonicus | |
22.8 | - |
L-arginine | mutant enzyme S282G | Apostichopus japonicus | |
25.7 | - |
ATP | wild-type enzyme | Apostichopus japonicus | |
25.7 | - |
L-arginine | wild-type enzyme | Apostichopus japonicus |