Crystallization (Comment) | Organism |
---|---|
crystal structures of mutant enzymes E314D at 1.9 A and E225Q at 2.8 A resolution shows that the precise alignment of substrates is subtly distorted | Limulus polyphemus |
Protein Variants | Comment | Organism |
---|---|---|
E225A | turnover number is 0.03% of the wild-type value | Limulus polyphemus |
E225D | KM-value for ADP is 2.2fold higher than the wild-type value, KM-value for N-phospho-L-arginine is 1.14fold higher than wild-type value, turnover number is 0.24% of the wild-type value | Limulus polyphemus |
E225Q | KM-value for ADP is 1.3fold higher than the wild-type value, KM-value for N-phospho-L-arginine is 1.4fold higher than wild-type value, turnover number is 0.3% of the wild-type value | Limulus polyphemus |
E225Q/E314Q | KM-value for ADP is 1.3fold higher than the wild-type value, KM-value for N-phospho-L-arginine is 2.3fold higher than wild-type value, turnover number is 0.2% of the wild-type value | Limulus polyphemus |
E314D | KM-value for ADP is 1.3fold higher than the wild-type value, KM-value for N-phospho-L-arginine is 1.56fold higher than wild-type value, turnover number is 1.7% of the wild-type value | Limulus polyphemus |
E314Q | KM-value for ADP is 1.2fold higher than the wild-type value, KM-value for N-phospho-L-arginine is 1.1fold higher than wild-type value, turnover number is 0.3% of the wild-type value | Limulus polyphemus |
R312G/E314V/H315D/E317A/E319V | KM-value for ADP is 1.5fold lower than the wild-type value, KM-value for N-phospho-L-arginine is 1.5fold higher than wild-type value, turnover number is 83% of the wild-type value | Limulus polyphemus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ADP | mutant enzyme R312G/E314V/H315D/E317A/E319V | Limulus polyphemus | |
0.14 | - |
ADP | mutant enzyme E314Q | Limulus polyphemus | |
0.16 | - |
ADP | mutant enzyme E225Q | Limulus polyphemus | |
0.16 | - |
ADP | mutant enzyme E225Q/E314Q | Limulus polyphemus | |
0.16 | - |
ADP | mutant enzyme E314S | Limulus polyphemus | |
0.23 | - |
ADP | wild-type enzyme | Limulus polyphemus | |
0.26 | - |
ADP | mutant enzyme E225D | Limulus polyphemus | |
0.63 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | wild-type enzyme | Limulus polyphemus | |
0.68 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E314D | Limulus polyphemus | |
0.72 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E225D | Limulus polyphemus | |
0.88 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E225Q | Limulus polyphemus | |
0.92 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme R312G/E314V/H315D/E317A/E319V | Limulus polyphemus | |
0.98 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E314S | Limulus polyphemus | |
1.45 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E225Q/E314Q | Limulus polyphemus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limulus polyphemus | - |
atlantic horseshoe crab | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + Nomega-phospho-L-arginine | - |
Limulus polyphemus | ATP + L-arginine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
ADP | mutant enzyme E225Q/E314Q | Limulus polyphemus | |
0.27 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E225Q/E314Q | Limulus polyphemus | |
0.34 | - |
ADP | mutant enzyme E225D | Limulus polyphemus | |
0.34 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E225D | Limulus polyphemus | |
0.37 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E314D | Limulus polyphemus | |
0.37 | - |
ADP | mutant enzyme E314Q | Limulus polyphemus | |
0.45 | - |
ADP | mutant enzyme E225Q | Limulus polyphemus | |
0.45 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E225Q | Limulus polyphemus | |
2.17 | - |
ADP | mutant enzyme E314D | Limulus polyphemus | |
2.17 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme E314S | Limulus polyphemus | |
116 | - |
ADP | mutant enzyme R312G/E314V/H315D/E317A/E319V | Limulus polyphemus | |
116 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | mutant enzyme R312G/E314V/H315D/E317A/E319V | Limulus polyphemus | |
140 | - |
ADP | wild-type enzyme | Limulus polyphemus | |
140 | - |
N5-(N-phosphonocarbamimidoyl)-L-ornithine | wild-type enzyme | Limulus polyphemus |