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Literature summary for 2.7.3.3 extracted from

  • Gattis, J.L.; Ruben, E.; Fenley, M.O.; Ellington, W.R.; Chapman, M.S.
    The active site cysteine of arginine kinase: structural and functional analysis of partially active mutants (2004), Biochemistry, 43, 8680-8689.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pleocyemata sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, the crystallographic structure of the alanine mutant at 2.3 A resolution, determined as a transition state analogue complex with arginine, nitrate, and MgADP2-, is nearly identical to wild type structure Pleocyemata sp.

Protein Variants

Protein Variants Comment Organism
C271A 1300fold decrease in turnover number, in presence of 1 mM Cl-. 3.6fold increase in Km-value for N-phospho-L-arginine, 7.5fold increase in Km-value for ATP, in presence of 1 mM Cl- Pleocyemata sp.
C271D 86.7fold decrease in turnover number, in presence of 1 mM Cl-. 9.8fold increase in Km-value for N-phospho-L-arginine, 2.5fold increase in Km-value for ATP, in presence of 1 mM Cl- Pleocyemata sp.
C271N 4727fold decrease in turnover number, in presence of 1 mM Cl-. 2.7fold increase in Km-value for N-phospho-L-arginine, 4.3fold increase in Km-value for ATP, in presence of 1 mM Cl- Pleocyemata sp.
C271S 1486fold decrease in turnover number in presence of 1 mM Cl-. 16.8fold increase in Km-value for N-phospho-L-arginine, 5fold increase in Km-value for ATP in presence of 1 mM Cl- Pleocyemata sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pleocyemata sp.

Organism

Organism UniProt Comment Textmining
Pleocyemata sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pleocyemata sp.

Source Tissue

Source Tissue Comment Organism Textmining
tail muscle
-
Pleocyemata sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine
-
Pleocyemata sp. ADP + Nomega-phosphono-L-arginine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Pleocyemata sp.