Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pleocyemata sp. |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, the crystallographic structure of the alanine mutant at 2.3 A resolution, determined as a transition state analogue complex with arginine, nitrate, and MgADP2-, is nearly identical to wild type structure | Pleocyemata sp. |
Protein Variants | Comment | Organism |
---|---|---|
C271A | 1300fold decrease in turnover number, in presence of 1 mM Cl-. 3.6fold increase in Km-value for N-phospho-L-arginine, 7.5fold increase in Km-value for ATP, in presence of 1 mM Cl- | Pleocyemata sp. |
C271D | 86.7fold decrease in turnover number, in presence of 1 mM Cl-. 9.8fold increase in Km-value for N-phospho-L-arginine, 2.5fold increase in Km-value for ATP, in presence of 1 mM Cl- | Pleocyemata sp. |
C271N | 4727fold decrease in turnover number, in presence of 1 mM Cl-. 2.7fold increase in Km-value for N-phospho-L-arginine, 4.3fold increase in Km-value for ATP, in presence of 1 mM Cl- | Pleocyemata sp. |
C271S | 1486fold decrease in turnover number in presence of 1 mM Cl-. 16.8fold increase in Km-value for N-phospho-L-arginine, 5fold increase in Km-value for ATP in presence of 1 mM Cl- | Pleocyemata sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pleocyemata sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleocyemata sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pleocyemata sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tail muscle | - |
Pleocyemata sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Pleocyemata sp. | ADP + Nomega-phosphono-L-arginine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pleocyemata sp. |