Protein Variants | Comment | Organism |
---|---|---|
D62E | 3.3% of Vmax of recombinant wild-type enzyme, Km-value for L-Arg is 99% of that of the wild-type enzyme | Nautilus pompilius |
D62G | 0.6% of Vmax of recombinant wild-type enzyme | Nautilus pompilius |
R193G | 1.5% of Vmax of recombinant wild-type enzyme | Nautilus pompilius |
S63G | 5.1% of Vmax of recombinant wild-type enzyme, Km-value for L-Arg is 516% of that of the wild-type enzyme | Nautilus pompilius |
S63T | 0.3% of Vmax of recombinant wild-type enzyme | Nautilus pompilius |
Y68S | mutant enzyme without activity | Nautilus pompilius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
L-Arg | - |
Nautilus pompilius | |
0.67 | - |
L-Arg | 25°C, mutant enzyme D62G | Octopus vulgaris | |
0.68 | - |
L-Arg | - |
Nautilus pompilius | |
0.68 | - |
L-Arg | 25°C, recombinant wild-type enzyme | Octopus vulgaris | |
1.02 | - |
L-Arg | 25°C, native enzyme | Nautilus pompilius | |
1.74 | - |
L-Arg | 25°C, native enzyme | Sepioteuthis lessoniana | |
2.82 | - |
L-Arg | 25°C, native enzyme | Octopus vulgaris | |
3.45 | - |
L-Arg | - |
Nautilus pompilius | |
3.45 | - |
L-Arg | 25°C, mutant enzyme S63G | Octopus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
x * 40500, SDS-PAGE | Nautilus pompilius |
40500 | - |
x * 40500, SDS-PAGE | Octopus vulgaris |
40500 | - |
x * 40500, SDS-PAGE | Sepioteuthis lessoniana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nautilus pompilius | Q9NKV6 | - |
- |
Octopus vulgaris | Q9NKV5 | - |
- |
Sepioteuthis lessoniana | Q9NKV4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Nautilus pompilius |
- |
Octopus vulgaris |
- |
Sepioteuthis lessoniana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall muscle | - |
Nautilus pompilius | - |
body wall muscle | - |
Octopus vulgaris | - |
body wall muscle | - |
Sepioteuthis lessoniana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Nautilus pompilius | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-Arg | - |
Octopus vulgaris | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-Arg | - |
Sepioteuthis lessoniana | ADP + Nomega-phospho-L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40500, SDS-PAGE | Nautilus pompilius |
? | x * 40500, SDS-PAGE | Octopus vulgaris |
? | x * 40500, SDS-PAGE | Sepioteuthis lessoniana |