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Literature summary for 2.7.3.3 extracted from

  • Suzuki, T.; Fukuta, H.; Nagato, H.; Umekawa, M.
    Arginine kinase from Nautilus pompilius, a living fossil. Site-directed mutagenesis studies on the role of amino acid residues in the Guanidino specificity region (2000), J. Biol. Chem., 275, 23884-23890.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D62E 3.3% of Vmax of recombinant wild-type enzyme, Km-value for L-Arg is 99% of that of the wild-type enzyme Nautilus pompilius
D62G 0.6% of Vmax of recombinant wild-type enzyme Nautilus pompilius
R193G 1.5% of Vmax of recombinant wild-type enzyme Nautilus pompilius
S63G 5.1% of Vmax of recombinant wild-type enzyme, Km-value for L-Arg is 516% of that of the wild-type enzyme Nautilus pompilius
S63T 0.3% of Vmax of recombinant wild-type enzyme Nautilus pompilius
Y68S mutant enzyme without activity Nautilus pompilius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
L-Arg
-
Nautilus pompilius
0.67
-
L-Arg 25°C, mutant enzyme D62G Octopus vulgaris
0.68
-
L-Arg
-
Nautilus pompilius
0.68
-
L-Arg 25°C, recombinant wild-type enzyme Octopus vulgaris
1.02
-
L-Arg 25°C, native enzyme Nautilus pompilius
1.74
-
L-Arg 25°C, native enzyme Sepioteuthis lessoniana
2.82
-
L-Arg 25°C, native enzyme Octopus vulgaris
3.45
-
L-Arg
-
Nautilus pompilius
3.45
-
L-Arg 25°C, mutant enzyme S63G Octopus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
x * 40500, SDS-PAGE Nautilus pompilius
40500
-
x * 40500, SDS-PAGE Octopus vulgaris
40500
-
x * 40500, SDS-PAGE Sepioteuthis lessoniana

Organism

Organism UniProt Comment Textmining
Nautilus pompilius Q9NKV6
-
-
Octopus vulgaris Q9NKV5
-
-
Sepioteuthis lessoniana Q9NKV4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nautilus pompilius
-
Octopus vulgaris
-
Sepioteuthis lessoniana

Source Tissue

Source Tissue Comment Organism Textmining
body wall muscle
-
Nautilus pompilius
-
body wall muscle
-
Octopus vulgaris
-
body wall muscle
-
Sepioteuthis lessoniana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Nautilus pompilius ADP + Nomega-phospho-L-Arg
-
?
ATP + L-Arg
-
Octopus vulgaris ADP + Nomega-phospho-L-Arg
-
?
ATP + L-Arg
-
Sepioteuthis lessoniana ADP + Nomega-phospho-L-Arg
-
?

Subunits

Subunits Comment Organism
? x * 40500, SDS-PAGE Nautilus pompilius
? x * 40500, SDS-PAGE Octopus vulgaris
? x * 40500, SDS-PAGE Sepioteuthis lessoniana