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Literature summary for 2.7.3.3 extracted from

  • Platzer, E.G.; Wang, W.; Thompson, S.N.; Borchardt, D.B.
    Arginine kinase and phosphoarginine, a functional phosphagen, in the rhabditoid nematode Steinernema carpocapsae (1999), J. Parasitol., 85, 603-607.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
L-Arg pH 7.5, 26°C Steinernema carpocapsae
0.42
-
ADP pH 7.5, 26°C Steinernema carpocapsae
0.73
-
Nomega-phospho-L-Arg pH 7.5, 26°C Steinernema carpocapsae
2.35
-
ATP pH 7.5, 26°C Steinernema carpocapsae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, Km-value: 0.476 mM, formation of ATP Steinernema carpocapsae

Organism

Organism UniProt Comment Textmining
Steinernema carpocapsae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
Steinernema carpocapsae
-
larva third-stage juvenile Steinernema carpocapsae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00227
-
formation of arginine phosphate, enzyme from crude extract Steinernema carpocapsae
0.00312
-
formation of ATP, enzyme from crude extract Steinernema carpocapsae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Steinernema carpocapsae ADP + Nomega-phospho-L-Arg
-
r
ATP + L-canavanine 7.3% of the activity with L-Arg Steinernema carpocapsae ADP + L-phosphocanavanine
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
formation of ATP, enzyme from third stage juveniles Steinernema carpocapsae
7.3
-
formation of ATP, enzyme from adult Steinernema carpocapsae
7.8
-
formation of arginine phosphate, enzyme from third-stage juveniles Steinernema carpocapsae
7.9 8.5 formation of arginine phosphate, enzyme from adult Steinernema carpocapsae