Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | product inhibition, competitive with ADP, noncompetitive with L-Arg | Apis mellifera | |
D-Arg | product inhibition, competitive with arginine phosphate and noncompetitive withg ADP | Apis mellifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Apis mellifera | |
Co2+ | activates | Apis mellifera | |
Cu2+ | activates | Apis mellifera | |
Mg2+ | highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP | Apis mellifera | |
Mg2+ | highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate | Apis mellifera | |
Mn2+ | less effective than Mg2+ in activation | Apis mellifera | |
Mn2+ | 4 mM, activation | Apis mellifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Apis mellifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Apis mellifera |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-arginine = ADP + Nomega-phospho-L-arginine | rapid equilibrium random mechanism | Apis mellifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Apis mellifera | ADP + Nomega-phospho-L-Arg | - |
r | |
ATP + L-arginine methyl ester | - |
Apis mellifera | ADP + Nomega-phospho-L-arginine methyl ester | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
synthesis of ATP and synthesis of N-phospho-L-Arg | Apis mellifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.2 | synthesis of ATP | Apis mellifera |
8.3 | - |
synthesis of N-phospho-L-arginine | Apis mellifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.2 | pH 5.0: about 30% of maximal activity, pH 8.2: about 30% of maximal activity, ATP synthesis | Apis mellifera |
6.3 | 9 | pH 6.3: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg | Apis mellifera |