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Literature summary for 2.7.3.3 extracted from

  • Cheung, A.C.
    Kinetic properties of arginine phosphokinase from honeybees, Apis mellifera L. (Hymenoptera, Apidae) (1973), Arch. Biochem. Biophys., 154, 28-39.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP product inhibition, competitive with ADP, noncompetitive with L-Arg Apis mellifera
D-Arg product inhibition, competitive with arginine phosphate and noncompetitive withg ADP Apis mellifera

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Apis mellifera
Co2+ activates Apis mellifera
Cu2+ activates Apis mellifera
Mg2+ highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP Apis mellifera
Mg2+ highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate Apis mellifera
Mn2+ less effective than Mg2+ in activation Apis mellifera
Mn2+ 4 mM, activation Apis mellifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
gel filtration Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Apis mellifera

Reaction

Reaction Comment Organism Reaction ID
ATP + L-arginine = ADP + Nomega-phospho-L-arginine rapid equilibrium random mechanism Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Apis mellifera ADP + Nomega-phospho-L-Arg
-
r
ATP + L-arginine methyl ester
-
Apis mellifera ADP + Nomega-phospho-L-arginine methyl ester
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
synthesis of ATP and synthesis of N-phospho-L-Arg Apis mellifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.2 synthesis of ATP Apis mellifera
8.3
-
synthesis of N-phospho-L-arginine Apis mellifera

pH Range

pH Minimum pH Maximum Comment Organism
5 8.2 pH 5.0: about 30% of maximal activity, pH 8.2: about 30% of maximal activity, ATP synthesis Apis mellifera
6.3 9 pH 6.3: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg Apis mellifera