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Literature summary for 2.7.3.3 extracted from

  • Rosenthal, G.A.; Dahlman, D.L.; Robinson, G.W.
    L-Arginine kinase from tobacco hornworm, Manduca sexta (L.). Purification, properties, and interaction with L-canavanine (1977), J. Biol. Chem., 252, 3679-3683.
    View publication on PubMed
Search for more literature for 2.7.3.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 ADP 37°C Manduca sexta
0.45
-
 ADP ADP in form of MgADP- Manduca sexta
0.5
-
 L-Arg 7°C Manduca sexta
0.7
-
 Nomega-phospho-L-Arg 37°C Manduca sexta
22
-
 L-canavanine 37°C Manduca sexta
27
-
 L-phosphocanavanine 37°C Manduca sexta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 sedimentation equilibrium centrifugation Manduca sexta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Arg Manduca sexta production of high-energy reserves N-phospho-L-Arg in insect muscles ADP + Nomega-phospho-L-Arg
-
 ?

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Manduca sexta

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Manduca sexta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
248
-
-
 Manduca sexta

Storage Stability

Storage Stability Organism
4°C, slow loss of activity during prolonged storage, can partially be reactivated by addition of 0.1% w/v 2-mercaptoethanol Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + Nomega-phospho-L-arginine
-
 Manduca sexta ATP + L-arginine
-
 r
ATP + L-Arg
-
 Manduca sexta ADP + Nomega-phospho-L-Arg
-
 r
ATP + L-Arg production of high-energy reserves N-phospho-L-Arg in insect muscles Manduca sexta ADP + Nomega-phospho-L-Arg
-
 ?
ATP + L-canavanine
-
 Manduca sexta ADP + L-phosphocanavanine
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Manduca sexta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.3
-
 L-(+)-(S)-canavanine
-
 Manduca sexta
50.7
-
 L-phosphocanavanine
-
 Manduca sexta
139
-
 L-Arg
-
 Manduca sexta
431
-
 Nomega-phospho-L-Arg
-
 Manduca sexta