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Literature summary for 2.7.3.2 extracted from

  • Zou, L.; Su, W.; Wang, M.; Huang, W.; Zhao, H.; Zhang, E.; Jin, J.; Xu, H.; Xiao, F.
    Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli (2017), Appl. Microbiol. Biotechnol., 101, 5639-5644 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-
mitochondrion
-
Homo sapiens 5739
-
plasma membrane
-
Homo sapiens 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphocreatine Homo sapiens
-
ATP + creatine
-
r
ATP + creatine Homo sapiens
-
ADP + phosphocreatine
-
?
ATP + creatine Homo sapiens
-
ADP + phosphocreatine
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens B2R892
-
-
Homo sapiens P06732 and P12277
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and His GraviTrap column chromatography Homo sapiens
Ni-NTA resin affinity column chromatography, His GraviTrap column chromatography, and streptavidin magnetic resin chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
cardiac muscle
-
Homo sapiens
-
skeletal muscle
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-80°C, TAP-purified recombinant isoform CK-MB in 50% (v/v) glycerol, 64 weeks, no loss of activity Homo sapiens
-80°C, all the samples with 500, 250, and 20 U/l enzyme activity or with 300, 100, and 20 ng/ml mass concentration in 50% (v/v) glycerol, 64 weeks, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphocreatine
-
Homo sapiens ATP + creatine
-
r
ATP + creatine
-
Homo sapiens ADP + phosphocreatine
-
?
ATP + creatine
-
Homo sapiens ADP + phosphocreatine
-
r

Subunits

Subunits Comment Organism
heterodimer
-
Homo sapiens
heterodimer 1 * 47200 + 1 * 43600, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
CK-MB isozyme Homo sapiens
CK-MB isoform Homo sapiens
creatine kinase-MB isozyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens