Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
plasma membrane | - |
Homo sapiens | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphocreatine | Homo sapiens | - |
ATP + creatine | - |
r | |
ATP + creatine | Homo sapiens | - |
ADP + phosphocreatine | - |
? | |
ATP + creatine | Homo sapiens | - |
ADP + phosphocreatine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | B2R892 | - |
- |
Homo sapiens | P06732 and P12277 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and His GraviTrap column chromatography | Homo sapiens |
Ni-NTA resin affinity column chromatography, His GraviTrap column chromatography, and streptavidin magnetic resin chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
cardiac muscle | - |
Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-80°C, TAP-purified recombinant isoform CK-MB in 50% (v/v) glycerol, 64 weeks, no loss of activity | Homo sapiens |
-80°C, all the samples with 500, 250, and 20 U/l enzyme activity or with 300, 100, and 20 ng/ml mass concentration in 50% (v/v) glycerol, 64 weeks, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphocreatine | - |
Homo sapiens | ATP + creatine | - |
r | |
ATP + creatine | - |
Homo sapiens | ADP + phosphocreatine | - |
? | |
ATP + creatine | - |
Homo sapiens | ADP + phosphocreatine | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Homo sapiens |
heterodimer | 1 * 47200 + 1 * 43600, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CK-MB | isozyme | Homo sapiens |
CK-MB | isoform | Homo sapiens |
creatine kinase-MB | isozyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |