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Literature summary for 2.7.3.2 extracted from
- Effect of Cd2+ on muscle type of creatine kinase: inhibition kinetics integrating computational simulations (2016), Int. J. Biol. Macromol., 83, 233-241.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | Cd2+ conspicuously inactivates the activity of the muscle-type enzyme in a first-order kinetic process and exhibits non-competitive inhibition with creatine and ATP. Cd2+ induces tertiary structure changes in enzyme PSCKM with exposure of hydrophobic surfaces. The addition of osmolytes, such as glycine and proline, partially reactivates the enzyme. Molecular dynamics and docking simulations between PSCKM and Cd2+ show that Cd2+ blocks the entrance of ATP to the active site of the enzyme, computational modeling, overview | Pelodiscus sinensis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.729 | - |
ATP | pH 9.0, 20°C | Pelodiscus sinensis | |
| 26.38 | - |
Creatine | pH 9.0, 20°C | Pelodiscus sinensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pelodiscus sinensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Pelodiscus sinensis | - |
ADP + phosphocreatine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelodiscus sinensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from muscle by anion exchange chromatography and gel filtration | Pelodiscus sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | muscle-type creatine kinase | Pelodiscus sinensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Pelodiscus sinensis | ADP + phosphocreatine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PSCKM | - |
Pelodiscus sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Pelodiscus sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Pelodiscus sinensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pelodiscus sinensis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inactivation rate constants for enzyme PSCKM by Cd2+., overview | Pelodiscus sinensis | |
| 0.423 | - |
Cd2+ | versus ATP, pH 9.0, 20°C | Pelodiscus sinensis | |
| 0.549 | - |
Cd2+ | versus creatine, pH 9.0, 20°C | Pelodiscus sinensis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
pH 9.0, 20°C | Pelodiscus sinensis | Cd2+ | |
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