Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | transition state analogue complex substrates inhibit the dimeric but not the octameric enzyme; transition state analogue complex substrates inhibit the dimeric but not the octameric enzyme | Clupea harengus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | there are two mitochondrial isoforms of CK: s-type (sarcomeric, expressed in skeletal and heart muscles only) and u-type (ubiquitous, expressed in many other tissues), which both exist as octamers in fish as well as in higher vertebrates and invertebrates. The mitochondrial s-type, rather than u-type, is predominantly expressed in herring eye | Clupea harengus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Clupea harengus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
dimeric enzyme form, gel filtration | Clupea harengus |
335000 | - |
octameric enzyme form of isozyme MtCK, gel filtration | Clupea harengus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | Clupea harengus | - |
ADP + phosphocreatine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clupea harengus | - |
isozyme uMtCK | - |
Clupea harengus | A0A0A1IVI8 | muscle-type CK; isozyme sMtCK, muscle-type CK, gene ckm | - |
Purification (Comment) | Organism |
---|---|
isolation of the octameric enzyme form from eyes by gel filtration, and affinity and anion exchange chromatgraphy | Clupea harengus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | four CK isoforms: one mitochondrial MtCK and three cytosolic isoforms: muscle-type CK (cyt-MM-CK), brain-type CK (cyt-BB-CK) and muscle-brain-type CK (cyt-MB-CK). The mitochondrial s-type, rather than u-type, is predominantly expressed in herring eye | Clupea harengus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
181 | - |
purified native octameric enzyme, pH and temperature not specified in the publication | Clupea harengus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Clupea harengus | ADP + phosphocreatine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | cytosolic isozyme | Clupea harengus |
More | MtCK generally exists as an octamer, but a dynamic octamer/dimer equilibrium exists in vitro depending on various parameters like MtCK concentration, temperatures and pH. Enzyme activity and temperature stability /sensitivity are higher for the octameric enzyme than for the dimeric enzyme. MtCK dimers are very sensitive and inactivate after exposure to temperatures above 30°C | Clupea harengus |
octamer | mitochondrial isozyme MtCK | Clupea harengus |
Synonyms | Comment | Organism |
---|---|---|
s-type CK | - |
Clupea harengus |
sarcomeric CK | - |
Clupea harengus |
sMtCK | - |
Clupea harengus |
u-type CK | - |
Clupea harengus |
ubiquitous CK | - |
Clupea harengus |
uMtCK | - |
Clupea harengus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | - |
Clupea harengus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | activity range, profile overview | Clupea harengus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity and temperature stability /sensitivity are higher for the octameric enzyme than for the dimeric enzyme. MtCK dimers are very sensitive and inactivate after exposure to temperatures above 30°C | Clupea harengus |
- |
60 | the enzyme acctivity is stable from 0°C up to 45°C, inactivation at 65°C | Clupea harengus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Clupea harengus | |
ATP | - |
Clupea harengus |
General Information | Comment | Organism |
---|---|---|
evolution | the MtCK gene is likely basal and ancestral and has evolved very early in metazoan evolution | Clupea harengus |