Literature summary for 2.7.3.2 extracted from

Fan, Y.Q.; Liu, H.J.; Li, C.; Luan, Y.S.; Yang, J.M.; Wang, Y.L.
Inactivation of recombinant human brain-type creatine kinase during denaturation by guanidine hydrochloride in a macromolecular crowding system (2013), Appl. Biochem. Biotechnol., 169, 268-280.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

General Stability

General Stability	Organism
polyethylene glycol 2000 (PEG 2000) and dextran 70 are used as model crowding agents to examine the effects of macromolecular crowding on the inactivation of recombinant HBCK (rHBCK) during denaturation by GdnHCl. Both PEG 2000 and dextran 70 have a protective effect on the inactivation of rHBCK induced by GdnHCl at 25°C. The presence of PEG 2000 results in the retention of 35.33% of rHBCK activity after 4 h of inactivation, while no rHBCK activity is observed after denaturation in the absence of macromolecular crowding agents. The presence of PEG 2000 and dextran 70 at a concentration of 100 g/l can decelerate the k2 value of the slow track to 21 and 33%, respectively, in comparison to values obtained in the absence of crowding agents	Homo sapiens

Organism

polyethylene glycol 2000 (PEG 2000) and dextran 70 are used as model crowding agents to examine the effects of macromolecular crowding on the inactivation of recombinant HBCK (rHBCK) during denaturation by GdnHCl. Both PEG 2000 and dextran 70 have a protective effect on the inactivation of rHBCK induced by GdnHCl at 25°C. The presence of PEG 2000 results in the retention of 35.33% of rHBCK activity after 4 h of inactivation, while no rHBCK activity is observed after denaturation in the absence of macromolecular crowding agents. The presence of PEG 2000 and dextran 70 at a concentration of 100 g/l can decelerate the k2 value of the slow track to 21 and 33%, respectively, in comparison to values obtained in the absence of crowding agents

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphocreatine	-	Homo sapiens	ATP + creatine	-	?

Synonyms

Synonyms	Comment	Organism
recombinant human brain-type creatine kinase	-	Homo sapiens
rHBCK	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens