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Literature summary for 2.7.3.2 extracted from

  • He, H.W.; Feng, S.; Pang, M.; Zhou, H.M.; Yan, Y.B.
    Role of the linker between the N- and C-terminal domains in the stability and folding of rabbit muscle creatine kinase (2007), Int. J. Biochem. Cell Biol., 39, 1816-1827.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
L115D gradual decrease in enzyme activity and secondary structures, mutation does not affect enzyme inactivation by heat or guanidine hydrochloride. Inactivated mutant cannot recover activity by dilution-initiated refolding Oryctolagus cuniculus
L121D gradual decrease in enzyme activity and secondary structures, mutation does not affect enzyme inactivation by heat or guanidine hydrochloride. Inactivated mutant cannot recover activity by dilution-initiated refolding Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
ATP mutant L115D Oryctolagus cuniculus
0.33
-
ATP mutant L110D Oryctolagus cuniculus
0.35
-
ATP mutant L121D Oryctolagus cuniculus
0.41
-
ATP wild-type Oryctolagus cuniculus
2.8
-
Creatine mutant L110D Oryctolagus cuniculus
3.1
-
Creatine mutant L115D Oryctolagus cuniculus
3.6
-
Creatine mutant L121D Oryctolagus cuniculus
8.7
-
Creatine wild-type Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P00563
-
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.5
-
mutant L115D Oryctolagus cuniculus
94.4
-
mutant L121D Oryctolagus cuniculus
276
-
wild-type Oryctolagus cuniculus
299
-
mutant L110D Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine
-
Oryctolagus cuniculus ADP + creatine phosphate
-
?

Subunits

Subunits Comment Organism
More mutants L110D, L115D, L121D are faster on SDS-PAGE than wild-type protein Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51.9
-
melting temperature of mutant L110D Oryctolagus cuniculus
52.3
-
melting temperature of wild-type Oryctolagus cuniculus
52.9
-
melting temperature of mutant L121D Oryctolagus cuniculus
53.6
-
melting temperature of mutant L115D Oryctolagus cuniculus