Literature summary for 2.7.3.2 extracted from

Hahn, H.S.; Park, Y.D.; Lee, J.R.; Park, K.H.; Kim, T.J.; Yang, J.M.; Hahn, M.J.
Aggregation and folding of recombinant human creatine kinase (2003), J. Protein Chem., 22, 563-570.

Search for more literature for 2.7.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, mostly in insoluble form, after optimization of the conditions the enzyme does not aggregate and shows multi-kinetic phases	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	prevents aggregation of the enzyme during recombinant expression in Escherichia coli	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
insoluble recombinant enzyme from Escherichia coli by 6 M urea, unfolding shows biphasic kinetic courses, aggregation during refolding follows first-order kinetics, refolding intermediates are stabilized by NaCl, refolded enzyme shows high specific activity	Homo sapiens

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Release 2023.1 (January 2023)