Cloned (Comment) | Organism |
---|---|
expression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
R129A | site-directed mutagenesis, inactive mutant | Oryctolagus cuniculus |
R129K | site-directed mutagenesis, very highly reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R131A | site-directed mutagenesis, inactive mutant | Oryctolagus cuniculus |
R131K | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R235A | site-directed mutagenesis, inactive mutant | Oryctolagus cuniculus |
R235K | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R291K | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R319K | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R340A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R340K | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
R340Q | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes | Oryctolagus cuniculus | |
0.7 | - |
ATP | pH 9.0, 30°C, recombinant wild-type enzyme | Oryctolagus cuniculus | |
0.7 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
1.1 | - |
ATP | pH 9.0, 30°C, recombinant mutant R340K | Oryctolagus cuniculus | |
1.1 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
1.6 | - |
ATP | pH 9.0, 30°C, recombinant mutant R291K | Oryctolagus cuniculus | |
1.6 | - |
ATP | pH 9.0, 30°C, recombinant mutant R340A | Oryctolagus cuniculus | |
1.6 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
3.6 | - |
ATP | pH 9.0, 30°C, recombinant mutant R235K | Oryctolagus cuniculus | |
3.6 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
7.3 | - |
ATP | pH 9.0, 30°C, recombinant mutant R340Q | Oryctolagus cuniculus | |
7.3 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
8.2 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R340A | Oryctolagus cuniculus | |
9 | - |
Creatine | pH 9.0, 30°C, recombinant wild-type enzyme | Oryctolagus cuniculus | |
10.3 | - |
ATP | pH 9.0, 30°C, recombinant mutant R129A | Oryctolagus cuniculus | |
10.3 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
19 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R340K | Oryctolagus cuniculus | |
72 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R235K | Oryctolagus cuniculus | |
76 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R291K | Oryctolagus cuniculus | |
163 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R340Q | Oryctolagus cuniculus | |
167 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R129A | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgATP2-, at 1 mM, dissociation constants of wild-type and mutant enzymes | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | Oryctolagus cuniculus | key enzyme in energy homeostasis | ADP + phosphocreatine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + creatine = ADP + phosphocreatine | bireactant catalytic mechanism, transition state stabilization by six arginines clustered in the active site of the enzyme | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Oryctolagus cuniculus | ADP + phosphocreatine | - |
r | |
ATP + creatine | key enzyme in energy homeostasis | Oryctolagus cuniculus | ADP + phosphocreatine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | pH 9.0, 30°C, recombinant mutant R129A | Oryctolagus cuniculus | |
0.08 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R129A | Oryctolagus cuniculus | |
0.08 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
0.54 | - |
ATP | pH 9.0, 30°C, recombinant mutant R235K | Oryctolagus cuniculus | |
0.54 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R235K | Oryctolagus cuniculus | |
0.54 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
1.33 | - |
ATP | pH 9.0, 30°C, recombinant mutant R340Q | Oryctolagus cuniculus | |
1.33 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R340Q | Oryctolagus cuniculus | |
1.33 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
2.14 | - |
ATP | pH 9.0, 30°C, recombinant mutant R340A | Oryctolagus cuniculus | |
2.14 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R340A | Oryctolagus cuniculus | |
2.14 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
2.18 | - |
ATP | pH 9.0, 30°C, recombinant mutant R291K | Oryctolagus cuniculus | |
2.18 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R291K | Oryctolagus cuniculus | |
2.18 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
50.1 | - |
ATP | pH 9.0, 30°C, recombinant mutant R340K | Oryctolagus cuniculus | |
50.1 | - |
Creatine | pH 9.0, 30°C, recombinant mutant R340K | Oryctolagus cuniculus | |
50.1 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus | |
148 | - |
ATP | pH 9.0, 30°C, recombinant wild-type enzyme | Oryctolagus cuniculus | |
148 | - |
Creatine | pH 9.0, 30°C, recombinant wild-type enzyme | Oryctolagus cuniculus | |
148 | - |
ATP | ATP in form of MgATP2- | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | MgADP2- | Oryctolagus cuniculus | |
ATP | MgATP2- | Oryctolagus cuniculus |