BRENDA - Enzyme Database
show all sequences of 2.7.3.1

Purification and properties of guanidinoacetate kinase from a polychaete, Perineis sp.

Shirokane, Y.; Nakajima, M.; Mizusawa, K.; Agric. Biol. Chem. 55, 2235-2242 (1991)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-guanidinopropionate
slight
Perinereis sp.
4-Guanidinobutyrate
slight
Perinereis sp.
5,5'-dithiobis(2-nitrobenzoate)
-
Perinereis sp.
agmatine
-
Perinereis sp.
AMP
-
Perinereis sp.
Ca2+
-
Perinereis sp.
Creatine
slight
Perinereis sp.
guanidine
slight
Perinereis sp.
Hg2+
-
Perinereis sp.
iodoacetamide
-
Perinereis sp.
L-arginine
slight
Perinereis sp.
methylguanidine
slight
Perinereis sp.
N-bromosuccinimide
-
Perinereis sp.
p-chloromercuribenzoate
-
Perinereis sp.
Tauroguanine
slight
Perinereis sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
ATP
pH 8.1, 25°C
Perinereis sp.
4.1
-
Guanidinoacetate
pH 8.1, 25°C
Perinereis sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
29% of Mg2+-activation
Perinereis sp.
Mg2+
5 mM, activation
Perinereis sp.
Mn2+
5 mM, activation
Perinereis sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
47000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
90000
-
gel filtration
Perinereis sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + guanidinoacetate
Perinereis sp.
-
ADP + phosphoguanidinoacetate
-
Perinereis sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Perinereis sp.
-
polychaete
-
Purification (Commentary)
Commentary
Organism
-
Perinereis sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
45.3
-
pH 8.1, 25°C
Perinereis sp.
Storage Stability
Storage Stability
Organism
-20°C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months
Perinereis sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + guanidinoacetate
-
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
ATP + guanidinoacetate
specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
Subunits
Subunits
Commentary
Organism
dimer
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
phosphorylation of guanidinoacetate
Perinereis sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
stable up to
Perinereis sp.
40
-
20 min, 4% loss of activity
Perinereis sp.
45
-
20 min, 28% loss of activity
Perinereis sp.
50
-
inactivation
Perinereis sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
phosphorylation of guanidinoacetate
Perinereis sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
less than 50% of maximal activity above and below
Perinereis sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.5
-
Perinereis sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-guanidinopropionate
slight
Perinereis sp.
4-Guanidinobutyrate
slight
Perinereis sp.
5,5'-dithiobis(2-nitrobenzoate)
-
Perinereis sp.
agmatine
-
Perinereis sp.
AMP
-
Perinereis sp.
Ca2+
-
Perinereis sp.
Creatine
slight
Perinereis sp.
guanidine
slight
Perinereis sp.
Hg2+
-
Perinereis sp.
iodoacetamide
-
Perinereis sp.
L-arginine
slight
Perinereis sp.
methylguanidine
slight
Perinereis sp.
N-bromosuccinimide
-
Perinereis sp.
p-chloromercuribenzoate
-
Perinereis sp.
Tauroguanine
slight
Perinereis sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
ATP
pH 8.1, 25°C
Perinereis sp.
4.1
-
Guanidinoacetate
pH 8.1, 25°C
Perinereis sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
29% of Mg2+-activation
Perinereis sp.
Mg2+
5 mM, activation
Perinereis sp.
Mn2+
5 mM, activation
Perinereis sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
47000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
90000
-
gel filtration
Perinereis sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + guanidinoacetate
Perinereis sp.
-
ADP + phosphoguanidinoacetate
-
Perinereis sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Perinereis sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
45.3
-
pH 8.1, 25°C
Perinereis sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months
Perinereis sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + guanidinoacetate
-
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
ATP + guanidinoacetate
specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
phosphorylation of guanidinoacetate
Perinereis sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
stable up to
Perinereis sp.
40
-
20 min, 4% loss of activity
Perinereis sp.
45
-
20 min, 28% loss of activity
Perinereis sp.
50
-
inactivation
Perinereis sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
phosphorylation of guanidinoacetate
Perinereis sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
less than 50% of maximal activity above and below
Perinereis sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.5
-
Perinereis sp.
Other publictions for EC 2.7.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721618
Lim
Structural basis for the mecha ...
Namalycastis sp.
Biochemistry
49
2031-2041
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
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1
-
1
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-
1
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1
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-
-
-
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-
-
-
-
-
661520
Mizuta
Isolation, characterization, a ...
Namalycastis sp.
Comp. Biochem. Physiol. B
140
387-393
2005
-
-
1
-
-
1
-
4
3
1
1
1
-
6
-
-
1
-
-
1
-
-
3
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
4
3
1
1
1
-
-
-
1
-
1
-
-
3
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
642369
Suzuki
Evolution of phosphagen kinase ...
Hediste diversicolor
J. Protein Chem.
18
13-19
1999
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-
1
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-
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3
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3
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1
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1
1
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1
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3
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1
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1
1
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-
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-
-
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-
-
-
-
642364
Shirokane
-
Purification and properties of ...
Perinereis sp.
Agric. Biol. Chem.
55
2235-2242
1991
-
-
-
-
-
-
15
2
-
3
3
1
-
1
-
-
1
-
-
-
1
1
2
1
1
-
4
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
15
-
2
-
3
3
1
-
-
-
1
-
-
1
1
2
1
1
-
4
-
1
1
1
-
-
-
-
-
-
-
642368
Furukohri
-
Preparation of glycocyamine ki ...
Perinereis brevicirris
Rep. USA Mar. Biol. Inst. Kochi Univ.
9
215ff
1987
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-
-
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1
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1
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1
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-
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-
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-
-
-
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-
642365
Pradel
Properties and amino acid comp ...
Nephtys caeca
Biochim. Biophys. Acta
154
305-314
1968
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
1
1
1
1
-
-
-
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-
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-
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-
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4
-
-
-
-
1
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642366
Pradel
-
On ATP:guanidinoacetate N-phos ...
Nephtys caeca
Biochim. Biophys. Acta
81
86-95
1964
-
-
-
-
-
-
6
4
-
3
-
-
-
1
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-
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-
1
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3
-
2
1
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2
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6
-
4
-
3
-
-
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-
1
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-
3
-
2
1
-
-
2
-
-
-
-
-
-
-
-
-
642367
Virden
Distribution of guanidine-aden ...
Myxicola infundibulum, Polycelis cornuta
Comp. Biochem. Physiol.
13
161-77
1964
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2
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2
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4
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2
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4
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