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Literature summary for 2.7.2.8 extracted from

  • Zhao, Q.; Luo, Y.; Dou, W.; Zhang, X.; Zhang, X.; Zhang, W.; Xu, M.; Geng, Y.; Rao, Z.; Xu, Z.
    Controlling the transcription levels of argGH redistributed L-arginine metabolic flux in N-acetylglutamate kinase and ArgR-deregulated Corynebacterium crenatum (2016), J. Ind. Microbiol. Biotechnol., 43, 55-66 .
    View publication on PubMed
Search for more literature for 2.7.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Corynebacterium crenatum

Protein Variants

Protein Variants Comment Organism
H268N the mutation significantly decreases the sensitivity of the enzyme to L-arginine and keeps the specific activity as compared to the wild type enzyme Corynebacterium crenatum
R209A the mutation significantly decreases the sensitivity of the enzyme to L-arginine and keeps the specific activity as compared to the wild type enzyme Corynebacterium crenatum

Inhibitors

Inhibitors Comment Organism Structure
L-arginine feedback inhibition, almost complete inhibition at 10 mM Corynebacterium crenatum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 40 mM used in assay conditions Corynebacterium crenatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-L-glutamate Corynebacterium crenatum
-
 ADP + N-acetyl-L-glutamyl 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate Corynebacterium crenatum SYPA5-5
-
 ADP + N-acetyl-L-glutamyl 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium crenatum
-
-
-
Corynebacterium crenatum SYPA5-5
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
 wild type enzyme, at pH 8.0 and 37°C Corynebacterium crenatum
0.77
-
 mutant enzyme R209A, at pH 8.0 and 37°C Corynebacterium crenatum
0.79
-
 mutant enzyme H268N, at pH 8.0 and 37°C Corynebacterium crenatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
 Corynebacterium crenatum ADP + N-acetyl-L-glutamyl 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate
-
 Corynebacterium crenatum SYPA5-5 ADP + N-acetyl-L-glutamyl 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
argB
-
 Corynebacterium crenatum
N-acetylglutamate kinase
-
 Corynebacterium crenatum
NagK
-
 Corynebacterium crenatum