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Literature summary for 2.7.2.8 extracted from

  • Zhao, Q.; Luo, Y.; Dou, W.; Zhang, X.; Zhang, X.; Zhang, W.; Xu, M.; Geng, Y.; Rao, Z.; Xu, Z.
    Controlling the transcription levels of argGH redistributed L-arginine metabolic flux in N-acetylglutamate kinase and ArgR-deregulated Corynebacterium crenatum (2016), J. Ind. Microbiol. Biotechnol., 43, 55-66.
    View publication on PubMed
Search for more literature for 2.7.2.8

Cloned(Commentary)

Cloned (Comment) Organism
gene argB, cloning in Escherichia coli strain JM109 and recombinant expression of wild-type and mutant enzymes in Corynebacterium crenatum strain SYPA5-5 Corynebacterium crenatum

Protein Variants

Protein Variants Comment Organism
A26V site-directed mutagenesis, the mutation deregulates the feedback inhibition of the enzyme Corynebacterium crenatum
E19R site-directed mutagenesis, the mutation increases the 50% inhibitoryL--arginine concentration significantly in vitro, which deregulates the feedback inhibition of CcNAGK by L-arginine in SYPA5-5 during the fermentation Corynebacterium crenatum
G287D site-directed mutagenesis, the mutation increases the 50% inhibitoryL--arginine concentration significantly in vitro, which deregulates the feedback inhibition of CcNAGK by L-arginine in SYPA5-5 during the fermentation Corynebacterium crenatum
H268N site-directed mutagenesis, the mutant strain shows accumulation of large amounts of pathway intermediates L-citrulline and L-ornithine and decreased production of L-arginine. Transcription levels of argGH decrease accompanied with the reduction of argininosuccinate synthase activity, which leads to the metabolic obstacle from L-citrulline to L-arginine Corynebacterium crenatum
H268N site-directed mutagenesis, the mutation increases the 50% inhibitoryL--arginine concentration significantly in vitro, which deregulates the feedback inhibition of CcNAGK by L-arginine in SYPA5-5 during the fermentation Corynebacterium crenatum
H26E site-directed mutagenesis, the mutation increases the 50% inhibitoryL--arginine concentration significantly in vitro, which deregulates the feedback inhibition of CcNAGK by L-arginine in SYPA5-5 during the fermentation Corynebacterium crenatum
M31V site-directed mutagenesis, the mutation deregulates the feedback inhibition of the enzyme Corynebacterium crenatum
additional information construction of feedback inhibition deregulated Corynebacterium crenatum mutant strains, phenotypes, overview Corynebacterium crenatum
R209A site-directed mutagenesis, the mutant strain shows accumulation of large amounts of pathway intermediates L-citrulline and L-ornithine and decreased production of L-arginine. Transcription levels of argGH decrease accompanied with the reduction of argininosuccinase activity, which leads to the metabolic obstacle from L-citrulline to L-arginine Corynebacterium crenatum
R209A site-directed mutagenesis, the mutation increases the 50% inhibitoryL--arginine concentration significantly in vitro, which deregulates the feedback inhibition of CcNAGK by L-arginine in SYPA5-5 during the fermentation Corynebacterium crenatum

Inhibitors

Inhibitors Comment Organism Structure
L-arginine feedback inhibition. SYPA5-5 N-acetylglutamate kinase feedback inhibition by L-arginine can be deregulated by introducing point mutations, H268N or R209A Corynebacterium crenatum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Corynebacterium crenatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-L-glutamate Corynebacterium crenatum
-
 ADP + N-acetyl-L-glutamyl 5-phosphate
-
 r
ATP + N-acetyl-L-glutamate Corynebacterium crenatum SYPA5-5
-
 ADP + N-acetyl-L-glutamyl 5-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Corynebacterium crenatum Q6R6P5 gene argB
-
Corynebacterium crenatum SYPA5-5 Q6R6P5 gene argB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
 Corynebacterium crenatum ADP + N-acetyl-L-glutamyl 5-phosphate
-
 r
ATP + N-acetyl-L-glutamate
-
 Corynebacterium crenatum SYPA5-5 ADP + N-acetyl-L-glutamyl 5-phosphate
-
 r

Synonyms

Synonyms Comment Organism
CcNAGK
-
 Corynebacterium crenatum
N-acetylglutamate kinase
-
 Corynebacterium crenatum
SYPA5-5 N-acetylglutamate kinase
-
 Corynebacterium crenatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Corynebacterium crenatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Corynebacterium crenatum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Corynebacterium crenatum

General Information

General Information Comment Organism
metabolism N-acetylglutamate synthase/kinase catalyzes a rate limiting step in L-arginine biosynthesis. The activity of the enzyme is allosterically regulated by L-arginine, The pathway and regulatory modes involved in l-arginine biosynthesis in Corynebacterium crenatum SYPA5-5, overview Corynebacterium crenatum