Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PII protein | - |
Synechococcus elongatus | |
PII protein | - |
Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Synechococcus elongatus |
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | complete inhibition at 0.5 mM | Arabidopsis thaliana | |
L-arginine | complete inhibition at 3-5 mM, PII-mediated relief from L-arginine inhibition is antagonized by 2-oxoglutarate | Arabidopsis thaliana | |
L-arginine | complete inhibition at 0.1-1.0 mM, PII-mediated relief from L-arginine inhibition is antagonized by 2-oxoglutarate | Synechococcus elongatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
0.4 | - |
ATP | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
0.6 | - |
ATP | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
0.85 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
0.87 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
1.1 | - |
ATP | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
3.1 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
7.4 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
85 | - |
N-acetyl-L-glutamate | in the presence of 1 mM L-arginine, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | Synechococcus elongatus | - |
ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
ATP + N-acetyl-L-glutamate | Arabidopsis thaliana | - |
ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SCL7 | - |
- |
Synechococcus elongatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Synechococcus elongatus |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Synechococcus elongatus | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
ATP + N-acetyl-L-glutamate | - |
Arabidopsis thaliana | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetyl-L-glutamate kinase | - |
Synechococcus elongatus |
N-acetyl-L-glutamate kinase | - |
Arabidopsis thaliana |
NagK | - |
Synechococcus elongatus |
NagK | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.1 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
40.4 | - |
N-acetyl-L-glutamate | in the presence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
115 | - |
N-acetyl-L-glutamate | in the presence of 1 mM L-arginine, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
126.1 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
187.5 | - |
N-acetyl-L-glutamate | in the presence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Synechococcus elongatus | |
ATP | - |
Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | L-arginine | |
0.19 | - |
in the presence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | L-arginine | |
1 | - |
in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | L-arginine | |
5.9 | - |
in the presence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | L-arginine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
N-acetyl-L-glutamate | in the presence of 1 mM L-arginine, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
1.7 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
13 | - |
N-acetyl-L-glutamate | in the presence of protein PII, in 50 mM imidazole, pH 7.5 | Synechococcus elongatus | |
145 | - |
N-acetyl-L-glutamate | in the absence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana | |
220 | - |
N-acetyl-L-glutamate | in the presence of protein PII, in 50 mM imidazole, pH 7.5 | Arabidopsis thaliana |