Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Pseudomonas aeruginosa | |
arginine | feedback inhibition is markedly dependent on pH, above pH 9 no inhibition | Pseudomonas aeruginosa | |
L-citrulline | - |
Pseudomonas aeruginosa | |
MgCl2 | 30 mM, slight inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
N-acetyl-L-glutamate | pH 7.2, 37°C | Pseudomonas aeruginosa | |
3 | - |
ATP | pH 7.2, 37°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ | Pseudomonas aeruginosa | |
Co2+ | Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ | Pseudomonas aeruginosa | |
Mg2+ | required | Pseudomonas aeruginosa | |
Mg2+ | 30 mM, slight inhibition | Pseudomonas aeruginosa | |
Mn2+ | Mn2+, Zn2+, Co2+ and Ca2+ in decreasing order can partially replace Mg2+ | Pseudomonas aeruginosa | |
Zn2+ | Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Pseudomonas aeruginosa | ADP + N-acetyl-L-glutamate 5-phosphate | in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate | ? | |
ATP + N-carbamoyl-L-glutamate | at 33% of the activity with N-acetyl-L-glutamate | Pseudomonas aeruginosa | ADP + N-carbamoyl-L-glutamate 5-phosphate | - |
? | |
ATP + N-formyl-L-glutamate | at 20% of the activity with N-acetyl-L-glutamate | Pseudomonas aeruginosa | ADP + N-formyl-L-glutamate 5-phosphate | - |
? | |
dATP + N-acetyl-L-glutamate | as effective as ATP | Pseudomonas aeruginosa | dADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
additional information | not: ITP | Pseudomonas aeruginosa | ? | - |
? | |
additional information | not: GTP | Pseudomonas aeruginosa | ? | - |
? | |
additional information | N-propionyl-L-glutamate almost inactive | Pseudomonas aeruginosa | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Pseudomonas aeruginosa |