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Literature summary for 2.7.2.8 extracted from

  • Haas, D.; Leisinger, T.
    N-acetylglutamate 5-phosphotransferase of Pseudomonas aeruginosa. Purification and ligand-directed association-dissociation (1975), Eur. J. Biochem., 52, 365-375.
    View publication on PubMed
Search for more literature for 2.7.2.8

General Stability

General Stability Organism
loss of activity on repeated freezing and thawing Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Pseudomonas aeruginosa
230000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-L-glutamate Pseudomonas aeruginosa enzyme synthesis not repressed by exogenous L-arginine or its precursors ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate Pseudomonas aeruginosa second enzyme of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-20°C, stable over extended periods Pseudomonas aeruginosa
4°C, 0.1 M phosphate buffer, stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
 Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate enzyme synthesis not repressed by exogenous L-arginine or its precursors Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate second enzyme of arginine biosynthesis Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Pseudomonas aeruginosa
More due to the capacity for self-association the enzyme can exist in different states of aggregation depending on the nature of the ligands and the concentration of phosphate buffer Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas aeruginosa