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Literature summary for 2.7.2.7 extracted from

  • Bachochin, M.J.; Venegas, J.C.; Singh, G.; Zhang, L.; Barber, R.D.
    Characterization of a butyrate kinase from Desulfovibrio vulgaris str. Hildenborough (2020), FEMS Microbiol. Lett., 367, fnaa047 .
    View publication on PubMed
Search for more literature for 2.7.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rosetta-2 cells Desulfovibrio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 ATP with butanoate as cosubstrate, at pH 7.5 and 37°C Desulfovibrio vulgaris
4.1
-
 Butanoate at pH 7.5 and 37°C Desulfovibrio vulgaris
6.7
-
 Isobutyrate at pH 7.5 and 37°C Desulfovibrio vulgaris
9.6
-
 valerate at pH 7.5 and 37°C Desulfovibrio vulgaris
11
-
 Isovalerate at pH 7.5 and 37°C Desulfovibrio vulgaris
14.3
-
 2-methylbutyrate at pH 7.5 and 37°C Desulfovibrio vulgaris
17.4
-
 propionate at pH 7.5 and 37°C Desulfovibrio vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 10 mM used in assay conditions Desulfovibrio vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94700
-
 gel filtration Desulfovibrio vulgaris

Organism

Organism UniProt Comment Textmining
Desulfovibrio vulgaris Q72EF0
-
-
Desulfovibrio vulgaris Hildenborough Q72EF0
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-ethylbutyrate 2.0% activity compared to valerate Desulfovibrio vulgaris ADP + 2-ethylbutyryl phosphate
-
 ?
ATP + 2-ethylbutyrate 2.0% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + 2-ethylbutyryl phosphate
-
 ?
ATP + 2-methylbutyrate 22.8% activity compared to valerate Desulfovibrio vulgaris ADP + 2-methylbutyryl phosphate
-
 ?
ATP + 2-methylbutyrate 22.8% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + 2-methylbutyryl phosphate
-
 ?
ATP + 2-methylpentanoate 7.8% activity compared to valerate Desulfovibrio vulgaris ADP + 2-methylpentanoyl phosphate
-
 ?
ATP + acetate 3.8% activity compared to valerate Desulfovibrio vulgaris ADP + acetyl phosphate
-
 ?
ATP + acetate 3.8% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + acetyl phosphate
-
 ?
ATP + butanoate 52.3% activity compared to valerate Desulfovibrio vulgaris ADP + butanoyl phosphate
-
 ?
ATP + butanoate 52.3% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + butanoyl phosphate
-
 ?
ATP + hexanoate 16.3% activity compared to valerate Desulfovibrio vulgaris ADP + hexanoyl phosphate
-
 ?
ATP + isobutyrate 43.1% activity compared to valerate Desulfovibrio vulgaris ADP + isobutyroyl phosphate
-
 ?
ATP + isovalerate 32.1% activity compared to valerate Desulfovibrio vulgaris ADP + isovaleroyl phosphate
-
 ?
ATP + propionate 60.8% activity compared to valerate Desulfovibrio vulgaris ADP + propionyl phosphate
-
 ?
ATP + propionate 60.8% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + propionyl phosphate
-
 ?
ATP + valerate 100% activity Desulfovibrio vulgaris ADP + valeroyl phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Desulfovibrio vulgaris

Synonyms

Synonyms Comment Organism
Buk
-
 Desulfovibrio vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
44
-
-
 Desulfovibrio vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44 60 at 44°C between pH 7.0 and 8.0, the enzyme remains stable and produces linear results up to 30 min under standard assay conditions. When subjected to a 15 min incubation at 60°C, the enzyme retains nearly 70% activity with 2.5 mM ATP and 30% activity with 50 mM butyrate Desulfovibrio vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.59
-
 2-methylbutyrate at pH 7.5 and 37°C Desulfovibrio vulgaris
2.18
-
 Isovalerate at pH 7.5 and 37°C Desulfovibrio vulgaris
2.94
-
 Isobutyrate at pH 7.5 and 37°C Desulfovibrio vulgaris
3.81
-
 Butanoate at pH 7.5 and 37°C Desulfovibrio vulgaris
4.35
-
 propionate at pH 7.5 and 37°C Desulfovibrio vulgaris
7.03
-
 ATP with butanoate as cosubstrate, at pH 7.5 and 37°C Desulfovibrio vulgaris
7.33
-
 valerate at pH 7.5 and 37°C Desulfovibrio vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Desulfovibrio vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
5 9 the enzyme demonstrates negligible activity below pH 5.0, while activity gradually decreased under conditions exceeding pH 8.0, maintaining about 60% of maximum activity at pH 9.0 Desulfovibrio vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 2-methylbutyrate at pH 7.5 and 37°C Desulfovibrio vulgaris
0.2
-
 Isovalerate at pH 7.5 and 37°C Desulfovibrio vulgaris
0.25
-
 propionate at pH 7.5 and 37°C Desulfovibrio vulgaris
0.44
-
 Isobutyrate at pH 7.5 and 37°C Desulfovibrio vulgaris
0.76
-
 valerate at pH 7.5 and 37°C Desulfovibrio vulgaris
0.93
-
 Butanoate at pH 7.5 and 37°C Desulfovibrio vulgaris
2.61
-
 ATP with butanoate as cosubstrate, at pH 7.5 and 37°C Desulfovibrio vulgaris