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Literature summary for 2.7.2.4 extracted from

  • Han, C.; Fang, L.; Liu, C.; Gao, Y.; Min, W.
    Construction of novel aspartokinase mutant A380I and its characterization by molecular dynamics simulation (2018), Molecules, 23, 3379 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.4

Protein Variants

Protein Variants Comment Organism
A380I the mutant has 11.32fold higher enzyme activity than the wild type enzyme, enhanced thermal stability and shows weakened inhibition with L-lysine Corynebacterium pekinense

Inhibitors

Inhibitors Comment Organism Structure
L-lysine 85.43% residual activity at 10 mM Corynebacterium pekinense
L-methionine 72.66% residual activity at 5 mM Corynebacterium pekinense
L-threonine 89.76% residual activity at 10 mM Corynebacterium pekinense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.66
-
 L-aspartate mutant enzyme A380I, at pH 8.0 and 28°C Corynebacterium pekinense
4.17
-
 L-aspartate wild type enzyme, at pH 8.0 and 28°C Corynebacterium pekinense

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.6 mM used in assay conditions Corynebacterium pekinense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate Corynebacterium pekinense
-
 ADP + 4-phospho-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium pekinense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, and Superdex 200 gel filtration Corynebacterium pekinense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Corynebacterium pekinense ADP + 4-phospho-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Corynebacterium pekinense

Synonyms

Synonyms Comment Organism
aspartokinase
-
 Corynebacterium pekinense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
-
 Corynebacterium pekinense

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 27 the enzyme shows more than 50% activity between 25°C and 27°C. At 20°C and 30°C, the enzyme exhibits about 40% and 50% activity, respectively Corynebacterium pekinense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Corynebacterium pekinense

pH Range

pH Minimum pH Maximum Comment Organism
7 9 the enzyme shows more than 50% activity between pH 7.0 and 9.0 Corynebacterium pekinense