Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
D45A | the inhibition of CgAK by lysine is substantially reduced in D45A mutant | Corynebacterium glutamicum |
S301F | the S301F mutant exhibits resistance to feedback inhibition by lysine and threonine, showing activity in the presence of both lysine and threonine | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | enzyme is inhibited by lysine and threonine in a concerted manner: comparison of the crystal structures between inhibitory and active forms reveal that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit | Corynebacterium glutamicum | |
L-threonine | enzyme is inhibited by lysine and threonine in a concerted manner: comparison of the crystal structures between inhibitory and active forms reveal that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Corynebacterium glutamicum | ADP + 4-phospho-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | crystal structure, heterotetramer composed of two alpha subunits and two beta subunits | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
CgAK | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Corynebacterium glutamicum |