Any feedback?
Literature summary for 2.7.2.4 extracted from
- A specialized aspartokinase enhances the biosynthesis of the osmoprotectants ectoine and hydroxyectoine in Pseudomonas stutzeri A1501 (2011), J. Bacteriol., 193, 4456-4468.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinantly expressed in Escherichia coli | Pseudomonas stutzeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-lysine | L-lysine inhibits Ask_LysC only in a mixture with L-threonine | Pseudomonas stutzeri |  |
| L-threonine | - |
Pseudomonas stutzeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
ATP | pH 8.0, 30°C | Pseudomonas stutzeri | |
| 3.8 | - |
ATP | pH 8.0, 30°C | Pseudomonas stutzeri | |
| 21.6 | - |
L-aspartate | pH 8.0, 30°C | Pseudomonas stutzeri | |
| 29.7 | - |
L-aspartate | pH 8.0, 30°C | Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
SDS-PAGE, recombinant protein, beta-subunit (amino acids 248-255) | Pseudomonas stutzeri |
| 44000 | - |
SDS-PAGE, recombinant protein | Pseudomonas stutzeri |
| 44300 | - |
calculated from cDNA | Pseudomonas stutzeri |
| 50000 | - |
SDS-PAGE, recombinant protein | Pseudomonas stutzeri |
| 52400 | - |
calculated from cDNA | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | - |
Pseudomonas stutzeri | ADP + 4-phospho-L-aspartate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ask_Ect | - |
Pseudomonas stutzeri |
| Ask_LysC | - |
Pseudomonas stutzeri |
| aspartokinase | - |
Pseudomonas stutzeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas stutzeri |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
pH 8.0, 30°C, mixture with L-lysine | Pseudomonas stutzeri | L-threonine | |
| 0.7 | - |
pH 8.0, 30°C, + 650 mM NaCl | Pseudomonas stutzeri | L-threonine | |
| 2.9 | - |
pH 8.0, 30°C | Pseudomonas stutzeri | L-threonine | |
| 3.6 | - |
pH 8.0, 30°C | Pseudomonas stutzeri | L-threonine | |
| 3.9 | - |
pH 8.0, 30°C, + 650 mM KCl | Pseudomonas stutzeri | L-threonine | |
| 13.5 | - |
pH 8.0, 30°C, + 650 mM KCl | Pseudomonas stutzeri | L-threonine | |
| 18.7 | - |
pH 8.0, 30°C, + 650 mM NaCl | Pseudomonas stutzeri | L-threonine | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
