Cloned (Comment) | Organism |
---|---|
into pET-41a expression vector and expression in Escherichia coli Rosetta (DE3) cells | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
by hanging-drop vapor-diffusion method, in the presence of L-aspartate and MgADP, to 2.822.69 A resolution, N-terminal catalytic domain (residues 2-300) and a C-terminal regulatory domain (residues 310-470) joined through a hinge region (residues 301-309) | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | - |
Methanocaldococcus jannaschii | |
additional information | L-lysine has no effect on activity at concentrations up to 10 mM, not inhibited by L-isoleucine and L-methionine | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stabilizes the negative charge density on the terminal phosphoryl group and lowers the barrier towards transfer to the beta-carboxyl group of L-aspartate | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
dynamic light-scattering, addition up to 4000 mM guanidine-HCl leads to dissociation of the AK dimers into monomers | Methanocaldococcus jannaschii |
83000 | - |
dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM causes a decrease in the AK particle size, dissociation into dimers | Methanocaldococcus jannaschii |
154000 | - |
dynamic light-scattering, crystallization | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57991 | - |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography and gel filtration, more than 99% pure | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Methanocaldococcus jannaschii | ADP + 4-phospho-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM | Methanocaldococcus jannaschii |
monomer | dynamic light-scattering, addition up to 4000 mM guanidine-HCl | Methanocaldococcus jannaschii |
tetramer | crystallography, dynamic light-scattering, organized into a dimer of dimers, dimer interface mediated through both ACT subdomains, formation of the tetramer stabilized by the interaction of complementary residues from alpha4 of the catalytic domain | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
AK | - |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 70 | - |
Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
L-aspartate | at 37°C | Methanocaldococcus jannaschii | |
245 | - |
L-aspartate | at 70°C | Methanocaldococcus jannaschii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.3 | - |
- |
Methanocaldococcus jannaschii | L-threonine |