Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-threonine | enhances activity up to 140% at 0.2 mM | Bacillus subtilis | |
L-threonine | enhances activity up to 140% at 0.2 mM | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
expression in Escherichia coli | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | no inhibition is observed; the truncated protein is no longer inhibited by lysine, even at 10 mM, wild type protein is inhibited by lysine | Bacillus subtilis | |
L-lysine | no inhibition is observed | Thermus thermophilus | |
L-threonine | almost insensitive to threonine | Bacillus subtilis | |
L-threonine | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
L-aspartate | - |
Bacillus subtilis | |
3.9 | - |
L-aspartate | - |
Thermus thermophilus | |
4.5 | - |
ATP | - |
Bacillus subtilis | |
4.5 | - |
ATP | - |
Thermus thermophilus | |
6.5 | - |
ATP | wild type | Bacillus subtilis | |
6.9 | - |
ATP | - |
Bacillus subtilis | |
6.9 | - |
ATP | - |
Thermus thermophilus | |
10 | - |
L-aspartate | wild type | Bacillus subtilis | |
10.6 | - |
L-aspartate | - |
Bacillus subtilis | |
10.6 | - |
L-aspartate | - |
Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
beta subunit, calculated from nucleotide sequence | Thermus thermophilus |
18000 | - |
alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence | Bacillus subtilis |
18500 | - |
beta subunit, calculated from nucleotide sequence | Bacillus subtilis |
42700 | - |
alpha subunit, calculated from nucleotide sequence | Bacillus subtilis |
42700 | - |
alpha subunit, calculated from nucleotide sequence | Thermus thermophilus |
43200 | - |
alpha subunit, calculated from nucleotide sequence | Thermus thermophilus |
43700 | - |
alpha subunit, calculated from nucleotide sequence | Bacillus subtilis |
43900 | - |
alpha subunit, calculated from nucleotide sequence | Bacillus subtilis |
43900 | - |
alpha subunit, calculated from nucleotide sequence | Thermus thermophilus |
60700 | - |
alphabeta, calculated from nucleotide sequence | Bacillus subtilis |
60700 | - |
alphabeta, calculated from nucleotide sequence | Thermus thermophilus |
61200 | - |
alphabeta, calculated from nucleotide sequence | Thermus thermophilus |
62200 | - |
alphabeta, calculated from nucleotide sequence | Bacillus subtilis |
64200 | - |
alpha2, gel filtration | Bacillus subtilis |
64200 | - |
alpha2, gel filtration | Thermus thermophilus |
87800 | - |
alpha2, calculated from nucleotide sequence | Bacillus subtilis |
87800 | - |
alpha2, calculated from nucleotide sequence | Thermus thermophilus |
90300 | - |
alpha2, beta2, gel filtration | Thermus thermophilus |
121400 | - |
alpha2, beta2, calculated from nucleotide sequence | Bacillus subtilis |
121400 | - |
alpha2, beta2, calculated from nucleotide sequence | Thermus thermophilus |
122400 | - |
alpha2, beta2, calculated from nucleotide sequence | Thermus thermophilus |
124400 | - |
alpha2, beta2, calculated from nucleotide sequence | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Bacillus subtilis | - |
ADP + phospho-L-aspartate | - |
? | |
ATP + L-aspartate | Thermus thermophilus | - |
ADP + phospho-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis | - |
chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus | - |
Bacillus subtilis | - |
chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus | - |
Thermus thermophilus | - |
- |
- |
Thermus thermophilus | - |
chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus | - |
Thermus thermophilus | - |
chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.38 | - |
truncated protein, which contains only the N-terminal region | Bacillus subtilis |
5.9 | - |
wild type | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Bacillus subtilis | ADP + phospho-L-aspartate | - |
? | |
ATP + L-aspartate | - |
Thermus thermophilus | ADP + phospho-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 43900, gel filtration, calculated from nucleotide sequence | Bacillus subtilis |
dimer | alpha2, 2 * 43900, gel filtration, calculated from nucleotide sequence | Thermus thermophilus |
tetramer | alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence | Bacillus subtilis |
tetramer | alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence | Thermus thermophilus |
tetramer | alpha2, beta2, 2 * 43200 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence | Thermus thermophilus |
tetramer | alpha2, beta2, 2 * 43700 + 2 * 18500, calculated from nucleotide sequence | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
AK | - |
Thermus thermophilus |
AK II | - |
Bacillus subtilis |
aspartate kinase | - |
Bacillus subtilis |
aspartate kinase | - |
Thermus thermophilus |
BT | - |
Bacillus subtilis |
BT | - |
Thermus thermophilus |
BTT | - |
Bacillus subtilis |
BTT | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
AK II loses activity very rapidly with a half-life of 31.5 s | Bacillus subtilis |
70 | - |
AK retains the full activity after 1 h of heat treatment | Thermus thermophilus |
70 | - |
loss of activity with a half-life of 45.7 s | Bacillus subtilis |
70 | - |
loss of activity with a half-life of 45.7 s | Thermus thermophilus |
70 | - |
no loss of activity even after 1 h of heat treatment | Bacillus subtilis |
70 | - |
no loss of activity even after 1 h of heat treatment | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.87 | - |
ATP | - |
Bacillus subtilis | |
11.87 | - |
ATP | - |
Thermus thermophilus | |
15.25 | - |
ATP | - |
Bacillus subtilis | |
15.25 | - |
ATP | - |
Thermus thermophilus | |
15.87 | - |
ATP | wild type | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.11 | - |
L-threonine | - |
Bacillus subtilis | |
1.11 | - |
L-threonine | - |
Thermus thermophilus | |
1.24 | - |
L-threonine | - |
Thermus thermophilus | |
1.28 | - |
L-lysine | - |
Bacillus subtilis |