Cloned (Comment) | Organism |
---|---|
single aspartate kinase encoded by the HOM3 gene, Escherichia coli DH5alpha as host | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A406T | site-directed mutagenesis, 30fold more strongly inhibited by threonine | Saccharomyces cerevisiae |
G25D | site-directed mutagenesis, reduced affinity for its substrates aspartate and ATP | Saccharomyces cerevisiae |
K26I | site-directed mutagenesis, reduced affinity for its substrates aspartate and ATP | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
ATP | mutant hom3-S45 | Saccharomyces cerevisiae | |
10.4 | - |
ATP | mutant hom3-S49 | Saccharomyces cerevisiae | |
11.3 | - |
L-aspartate | mutant hom3-S45 | Saccharomyces cerevisiae | |
12.4 | - |
L-aspartate | mutant hom3-S49 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Saccharomyces cerevisiae | first step in the branched pathway leading to synthesis of threonine and methionine from aspartate | ADP + 4-phospho-L-aspartate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Saccharomyces cerevisiae | ADP + 4-phospho-L-aspartate | - |
r | |
ATP + L-aspartate | first step in the branched pathway leading to synthesis of threonine and methionine from aspartate | Saccharomyces cerevisiae | ADP + 4-phospho-L-aspartate | - |
r |