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Literature summary for 2.7.2.4 extracted from

  • Keng, Y.F.; Viola, R.E.
    Specificity of aspartokinase III from Escherichia coli and an examination of important catalytic residues (1996), Arch. Biochem. Biophys., 335, 73-81.
    View publication on PubMed
Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment) Organism
lysC geneencoding for AKIII cloned into vector pTZ19U and expressed in the protease-deficient Escherichia coli BL21DE3 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
D-aspartate beta-hydroxamate
-
 Escherichia coli
D-aspartic acid 1-benzyl ester
-
 Escherichia coli
Diethyl aminomalonate
-
 Escherichia coli
diethyldicarbonate
-
 Escherichia coli
DL-3-amino-4-hydroxybutyrate methyl ester
-
 Escherichia coli
DL-3-aminobutyrate
-
 Escherichia coli
L-2-aminobutyrate competitive inhibitor Escherichia coli
L-glutamate
-
 Escherichia coli
L-glutamate gamma-methyl ester
-
 Escherichia coli
L-homoserine
-
 Escherichia coli
L-malate
-
 Escherichia coli
L-norvaline
-
 Escherichia coli
malonate
-
 Escherichia coli
N-Acetylimidazole
-
 Escherichia coli
N-ethylmaleimide
-
 Escherichia coli
succinate
-
 Escherichia coli
Tetranitromethane
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.5
-
 L-aspartate beta-benzyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
4.9
-
 L-aspartate beta-methyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
5.3
-
 L-aspartic acid 1-benzyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
13
-
 L-aspartate beta-hydroxamate pH 8.0, 25°C, aspartokinase III Escherichia coli
16
-
 L-asparagine pH 8.0, 25°C, aspartokinase III Escherichia coli
41
-
 N-formyl-L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
48
-
 N-acetyl-L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
68
-
 N-Chloroacetyl-L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
147
-
 DL-threo-3-methyl aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
184
-
 L-aspartic acid amide pH 8.0, 25°C, aspartokinase III Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
aspartokinase III Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + DL-threo-3-methyl aspartate aspartokinase III Escherichia coli ADP + 3-methyl-4-phosphoaspartate
-
 ?
ATP + L-asparagine aspartokinase III Escherichia coli ?
-
 ?
ATP + L-aspartate beta-hydroxamate aspartokinase III Escherichia coli ?
-
 ?
ATP + L-aspartate beta-methyl ester aspartokinase III Escherichia coli ?
-
 ?
ATP + L-aspartic acid 1-benzyl ester aspartokinase III Escherichia coli ?
-
 ?
ATP + L-aspartic acid 4-benzyl ester aspartokinase III Escherichia coli ?
-
 ?
ATP + L-aspartic acid amide aspartokinase III Escherichia coli ADP + 4-phospho-L-aspartic acid amide
-
 ?
ATP + N-acetyl-L-aspartate aspartokinase III Escherichia coli ADP + N-acetyl-4-phospho-L-aspartate
-
 ?
ATP + N-chloroacetyl-L-aspartate aspartokinase III Escherichia coli ADP + N-chloroacetyl-4-phospho-L-aspartate
-
 ?
ATP + N-formyl-L-aspartate aspartokinase III Escherichia coli ADP + N-formyl-4-phospho-L-aspartate
-
 ?
additional information aspartokinase III, D-isomers of the derivatives of aspartic acid, including D-aspartate alpha-benzyl ester and D-aspartate beta-hydroxamate are not substrates regardless of whether the alpha- or the beta-carboxyl group is derivatized, L-cysteine sulfinate and 2-methyl-DL-aspartate are no substrates Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000 Escherichia coli

Synonyms

Synonyms Comment Organism
aspartokinase III
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.7
-
 L-homoserine pH 8.0, 25°C, aspartokinase III Escherichia coli
14
-
 D-aspartic acid 1-benzyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
14
-
 DL-3-amino-4-hydroxybutyrate methyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
28
-
 L-2-aminobutyrate pH 8.0, 25°C, aspartokinase III Escherichia coli
32
-
 L-norvaline pH 8.0, 25°C, aspartokinase III Escherichia coli
53
-
 L-malate pH 8.0, 25°C, aspartokinase III Escherichia coli
63
-
 Diethyl aminomalonate pH 8.0, 25°C, aspartokinase III Escherichia coli
82
-
 L-glutamate gamma-methyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
87
-
 D-aspartate beta-hydroxamate pH 8.0, 25°C, aspartokinase III Escherichia coli
108
-
 malonate pH 8.0, 25°C, aspartokinase III Escherichia coli
128
-
 L-glutamate pH 8.0, 25°C, aspartokinase III Escherichia coli
159
-
 succinate pH 8.0, 25°C, aspartokinase III Escherichia coli
245
-
 DL-3-aminobutyrate pH 8.0, 25°C, aspartokinase III Escherichia coli