Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.4 extracted from

  • Dotson, S.B.; Somers, D.A.; Gengenbach, B.G.
    Purification and characterization of lysine-sensitive aspartate kinase from maize cell cultures (1989), Plant Physiol., 91, 1602-1608.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.4

Inhibitors

Inhibitors Comment Organism Structure
L-lysine
-
 Zea mays
L-Lysine ethyl ester
-
 Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Zea mays 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 2 * 49000 + 2 * 60000, SDS-PAGE Zea mays
60000
-
 2 * 49000 + 2 * 60000, SDS-PAGE Zea mays
113000
-
 gel filtration, native PAGE, isoenzyme AK Late Zea mays
255000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate Zea mays first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine ADP + 4-phospho-L-aspartate
-
 r

Organism

Organism UniProt Comment Textmining
Zea mays
-
 maize
-
Zea mays
-
 Black Mexican Sweet
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 18.9 units/mg Zea mays

Storage Stability

Storage Stability Organism
-70°C, stored in buffer containing 200 mM KCl and 2 mg/ml bovine serum albumin, stable for more than 1 year Zea mays
23°C, more than 90% stable for up to 1 week Zea mays
4°C, stable for more than 1 week Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Zea mays ADP + 4-phospho-L-aspartate
-
 r
ATP + L-aspartate first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine Zea mays ADP + 4-phospho-L-aspartate
-
 r

Subunits

Subunits Comment Organism
tetramer native AK Late isoform is a tetramer which dissociates into active dimers during native gradient PAGE Zea mays
tetramer 2 * 49000 + 2 * 60000, SDS-PAGE Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Zea mays