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Literature summary for 2.7.2.4 extracted from

  • Relton, J.M.; Bonner, P.L.R.; Wallsgrove, R.M.; Lea, P.J.
    Physical and kinetic properties of lysine-sensitive aspartate kinase purified from carrot cell suspension culture (1988), Biochim. Biophys. Acta, 953, 48-60.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
beta-Alanine 10% activation at 0.33 M Daucus carota

General Stability

General Stability Organism
stable up to. In the DEAE-Sephacel stage of purification, L-lysine and L-proline protect from heat inactivation Daucus carota

Inhibitors

Inhibitors Comment Organism Structure
HgSO4 15% inhibition Daucus carota
iodosalicylate 20% inhibition Daucus carota
L-lysine
-
Daucus carota
N-ethylmaleimide 17% inhibition Daucus carota
p-chloromercuribenzoate
-
Daucus carota
Rose bengal
-
Daucus carota
S-adenosyl-L-methionine potentiates inhibition by lysine Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
ATP pH 7.5, 30°C Daucus carota
2.35
-
L-aspartate pH 7.5, 30°C Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require monovalent cations for optimum activity Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
sucrose density gradient centrifugation, peak 1 Daucus carota
150000
-
sucrose density gradient centrifugation, peak 2 Daucus carota
230000
-
sucrose density gradient centrifugation, peak 3 Daucus carota
246000
-
gel filtration, Superose 6 Daucus carota
253000
-
gel filtration, non-denaturing electrophoresis, 4-20% polyacrylamide gradient gels Daucus carota
258000
-
gel filtration, Superose 12 Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
carrot
-

Purification (Commentary)

Purification (Comment) Organism
-
Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Daucus carota
-

Storage Stability

Storage Stability Organism
-20°C, can be stored without any noticeable loss in activity Daucus carota
use of bicine buffer and addition of sorbitol, bovine serum albumin or poly(ethyleneglycol) 6000 allows storage of the partially purified enzyme as a freeze-dried powder with relatively small loss of activity upon rehydration Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
Daucus carota ADP + 4-phospho-L-aspartate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Daucus carota

pI Value

Organism Comment pI Value Maximum pI Value
Daucus carota in presence of 0.1 mM L-lysine, determined by chromatofocusing
-
4.43
Daucus carota in absence of L-lysine, determined by chromatofocusing
-
5.16