Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | significantly stimulates dap-aspartokinase | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
feedback inhibitor can partially protect from heat inactivation up to 70°C | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-meso-diaminopimelic acid | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
L-aspartate | pH 8.0, 55°C, in presence of inhibitor | Geobacillus stearothermophilus | |
5 | - |
L-aspartate | pH 8.0, 55°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | significantly stimulates dap-aspartokinase | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
- |
Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Geobacillus stearothermophilus | - |
ADP + 4-phospho-L-aspartate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.046 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-20°C, storage of the enzyme in the frozen state followed by subsequent thawing leads to a rapid decline in activity | Geobacillus stearothermophilus |
4°C, enzyme activity can be maintained for several months | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Geobacillus stearothermophilus | ADP + 4-phospho-L-aspartate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
dap-aspartokinase | mesodiaminopimelate-sensitive isoenzyme | Geobacillus stearothermophilus |
LT-aspartokinase | lysine-threonine-sensitive isoenzyme | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
very rapid loss of catalytic activity above | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.1 | meso-diaminopimelate sensitive isoenzyme | Geobacillus stearothermophilus |