Protein Variants | Comment | Organism |
---|---|---|
E252A | specific activity (micromol/min/mg): 220 (wild-type: 420), Tm: 52.1°C, t1/2 (37°C): 7 months. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years | Homo sapiens |
I47N | specific activity (micromol/min/mg): 90 (wild-type: 420), Tm: 44.6°C, t1/2 (37°C): 27 min. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years | Homo sapiens |
L89P | specific activity (micromol/min/mg): 260 (wild-type: 420), Tm: 43°C, t1/2 (37°C): 13 min. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years | Homo sapiens |
T378P | specific activity (micromol/min/mg): 65 (wild-type: 420), Tm: 49.8°C, t1/2 (37°C): 3.9 days. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ion-exchange chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65 | - |
mutant T378P, pH 7.4, 25°C | Homo sapiens |
90 | - |
mutant I47N, pH 7.4, 25°C | Homo sapiens |
220 | - |
mutant E252A, pH 7.4, 25°C | Homo sapiens |
260 | - |
mutant L89P, pH 7.4, 25°C | Homo sapiens |
420 | - |
wild-type, pH 7.4, 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Homo sapiens | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGK1 | - |
Homo sapiens |
phosphoglycerate kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52.8 | - |
Tm (wild-type): 52.8°C, t1/2 (wild-type, 37°C): 3 years | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |