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Literature summary for 2.7.2.3 extracted from

  • Wu, H.; Chou, C.; Hung, Y.; Yu, C.
    Allotransplantation of transgenic mouse ovaries expressing enhanced green fluorescent protein under the control of the murine phosphoglycerate kinase 1 promoter (2010), Reprod. Domest. Anim., 45, 90090-6.
    View publication on PubMed

Application

Application Comment Organism
medicine transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP) under the control of the phosphoglycerate kinase 1 promoter by allotransplantation of transgenic mice ovaries may be useful for studies assessing the use of gene therapy of bone marrow or nerve cells, as well as for tumorigenesis and organ transplantation studies Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
1872-bp Pgk1/EGFP DNA fragment excised by double digestion of MluI and DraIII, consisting of 519 bp of the Pgk1 promoter, 723 bp of the EGFP cDNA and 224 bp of the bovine growth hormone (BGH) poly-A tail from the vector pPgk1/EGFP. Microinjection into the pronucleus of in vitro-fertilized FVB/NCrl mouse eggs and re-implanted into pseudopregnant ICR females to generate transgenic mice. CHO-K1 cells transfected with pPgk1/EGFP Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P09411
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-

Source Tissue

Source Tissue Comment Organism Textmining

Synonyms

Synonyms Comment Organism
3-phosphoglycerate kinase
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Mus musculus
PGK1
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Mus musculus
phosphoglycerate kinase 1
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Mus musculus

General Information

General Information Comment Organism
physiological function Pgk1/EGFP expression in ovaries is effective in protecting the fecundity of the partial ovaries after ovary allotransplantation Mus musculus