Protein Variants | Comment | Organism |
---|---|---|
K215A | kinetics of substrate binding and the associated protein isomerization steps are fast and identical for the wild-type and mutant K215A. Has a low inherent phosphotransferase activity, with the mutant the conserved Arg 65 replaces the missing Lys 215 by helping to position the transferable phospho group during the reaction. In the mutant the closed conformation of the enzyme is stabilized by a salt bridge between Asp 218 and Arg 170 rather than Arg 65 in the wild-type PGK | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
ATP | wild-type, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
0.077 | - |
ADP | wild-type, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
0.126 | - |
ADP | mutant K215A, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
1.6 | - |
ATP | mutant K215A, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate | - |
Homo sapiens | ADP + 3-phospho-D-glyceroyl 1-phosphate | - |
r | |
ADP + 3-phospho-D-glyceroyl 1-phosphate | - |
Homo sapiens | ATP + 3-phospho-D-glycerate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoglycerate kinase | - |
Homo sapiens |
PGK | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | mutant K215A, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
0.06 | - |
ADP | mutant K215A, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
0.48 | - |
ATP | mutant K215A, at 20°C, in methanol-free buffer, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
0.78 | - |
ADP | mutant K215A, at 20°C, in methanol-free buffer, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
2.48 | - |
ATP | wild-type, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
200 | - |
ADP | wild-type, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
833 | - |
ATP | wild-type, at 20°C, in methanol-free buffer, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
2633 | - |
ADP | wild-type, at 20°C, in methanol-free buffer, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ATP | mutant K215A, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
0.48 | - |
ADP | mutant K215A, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
150 | - |
ATP | wild-type, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens | |
2600 | - |
ADP | wild-type, at 4°C, in 30% methanol, 20 mM triethanolamine, pH 7.5, 0.1 M potassium acetate, and 1 mM free Mg2+ | Homo sapiens |