Crystallization (Comment) | Organism |
---|---|
crystallization from ammonium sulfate precipitation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
250fold from liver, to homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
143 | - |
purified enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Bos taurus | ADP + 1,3-diphosphoglycerate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
unstable below | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Bos taurus | |
ATP | required as phosphate donor | Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | 3 peaks of purified enzyme at pH 3.8, pH 5.75, and pH 7.45, isoelectric focusing using an analytical column with carrier ampholytes | - |
3.8 |
Bos taurus | 3 peaks of purified enzyme at pH 3.8, pH 5.75, and pH 7.45, isoelectric focusing using an analytical column with carrier ampholytes | - |
5.75 |
Bos taurus | 3 peaks of purified enzyme at pH 3.8, pH 5.75, and pH 7.45, isoelectric focusing using an analytical column with carrier ampholytes | - |
7.45 |